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S8160

Superoxide Dismutase from bovine liver

Name: Superoxide Dismutase from bovine liver
Brand: SIGMA
Price: 240 USD

lyophilized powder, ≥1500 units/mg protein

Sinónimos:

SOD, Superoxide: superoxide oxidoreductase

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A ustedes/SKU	Disponibilidad	Precio
15000 units	Comprobar disponibilidad del carrito	US$ 240,00
30000 units	Comprobar disponibilidad del carrito	US$ 414,00
75000 units	Comprobar disponibilidad del carrito	US$ 825,00

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Número CAS:

9054-89-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-943-0

MDL number:

MFCD00132404

Número CE:

1.15.1.1 (BRENDA, IUBMB)

Specific activity:

≥1500 units/mg protein

US$ 240,00

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Propiedades

form

lyophilized powder

Quality Segment

200

specific activity

≥1500 units/mg protein

mol wt

32.5 kDa

composition

Protein, ≥70% biuret

UniProt accession no.

P00442

storage temp.

−20°C

Gene Information

cow ... SOD1(281495)

Descripción

General description

Research area: Cell signalingSuperoxide Dismutase (SOD), a low molecular weight protein that is found in all aerobic cells of microorganisms, plants, and animals. It exists as three distinct families such as manganese SOD, copper–zinc SOD, and extracellular SOD.[1]

Application

Superoxide dismutase from bovine liver has been used in a study to determine that hypercholesterolemia increases endothelial superoxide anion production. [2] Superoxide dismutase from bovine liver has also been used in a study to investigate diazo coupling, subunit interactions, and electrophoretic variants of bovine erythrocyte superoxide dismutase. [3]It has also been used as a component of the assay buffer in thequantification of reactive oxygen species (ROS) by O2k fluorometry.[4]

Biochem/physiol Actions

Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Physical form

Lyophilized powder containing potassium phosphate buffer salts

Analysis Note

For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).

Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀of 0.025 ± 0.005 per min.

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Este artículo	S8409	S7571	S7446
Sigma-Aldrich S8160 Superoxide Dismutase from bovine liver	Sigma-Aldrich S8409 Superoxide Dismutase from bovine liver	Sigma-Aldrich S7571 Superóxido dismutasa from bovine erythrocytes	Sigma-Aldrich S7446 Superóxido dismutasa from bovine erythrocytes
description lyophilized powder, ≥1500 units/mg protein	description ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)	description lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret	description BioUltra, lyophilized powder, ≥4,500 units/mg protein, ≥97% (SDS-PAGE)
specific activity ≥1500 units/mg protein	specific activity 2,000-6,000 units/mg protein (biuret)	specific activity ≥3,000 units/mg protein	specific activity ≥4,500 units/mg protein
Gene Information cow ... SOD1(281495)	Gene Information cow ... SOD1(281495)	Gene Information cow ... SOD1(281495), SOD2(281496)	Gene Information -
form lyophilized powder	form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder
mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa	mol wt 32.5 kDa
UniProt accession no. P00442	UniProt accession no. P00442	UniProt accession no. P00442, P41976	UniProt accession no. -
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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