S9555
Anti-E-Selectin (CD62E) antibody, Mouse monoclonal
clone 1.2B6, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-CD62E, Anti-E-Selectin, Anti-ELAM-1
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| Size/SKU | Availability | Price |
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0.2 mL | Please contact Customer Service for Availability | $744.00 $632.40 |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
1.2B6, monoclonal
Application:
ELISA (c), ELISA (i), FACS, IF, IHC (f), IP, WB
Citations:
19
$632.40
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biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1.2B6, monoclonal
form
buffered aqueous solution
mol wt
antigen 100-115 kDa
species reactivity
human, pig
concentration
~2 mg/mL
technique(s)
capture ELISA: suitable, flow cytometry: suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1-2 μg/mL using using 293-T cells transfected with E-selectin fusion protein, western blot: suitable (non-reduced)
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SELE(6401)
General description
CD62E (E-Selectin) surface antigen is a glycoprotein and member of the selectin family of adhesion receptors. It is expressed by activated human endothelial cells and is detectable in the plasma.
Monoclonal anti-E-selectin (CD62E) (mouse IgG1 isotype) is derived from the 1.2B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with TNF-α activated human endothelial cells. This antibody reacts specifically with E-selectin (CD62E) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant human P-selectin and human platelet P-selectin. Cross-reactivity has been observed with porcine E-selectin.
It is a 140KDa protein mainly expressed in endothelial cells. E- selectin is characterized with an N-terminal domain, epidermal growth factor (EGF)-like domain, lectin (ligand-binding) domain, variable short consensus repeats and transmembrane domain.
It is a 140KDa protein mainly expressed in endothelial cells. E- selectin is characterized with an N-terminal domain, epidermal growth factor (EGF)-like domain, lectin (ligand-binding) domain, variable short consensus repeats and transmembrane domain.
Selectin E (SELE) is encoded by the gene mapped to human chromosome 1q24.2. The encoded protein is mainly expressed on luminal surfaces of inflamed vasculature.
Immunogen
TNF-α activated human endothelial cells.
Application
Anti-E-Selectin (CD62E) antibody, Mouse monoclonal has been used in immunofluorescence.
Biochem/physiol Actions
E-selectin acts as a key inflammatory cytokine. E-selectin-dependent rolling helps in binding pancreatic cancer cell to hyaluronic acid. In addition, the encoded protein in association with P-selectins, plays a vital role in recruiting leukocytes to inflammatory sites. Mutation in the gene is associated with the development of various diseases such as atherosclerosis and stroke.
Reacts specifically with human CD62E (E-Selectin, ELAM-1) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant hman P-Selectin and human platelet P-Selectin. Cross-reactivity has been observed with porcine E-Selectin. Monoclonal Anti-human E-Selctin inhibits adhesion of neutrophils, eosinophils, and a skin-homing T-cell subpopulation to activated endothelial cells and CD62E-transfected cells. 5th Workshop: code no. E 040.
Selectins play a vital role in maintaining homoeostasis and cutaneous inflammation. SEL-E and SEL-P help in carrying T helper (Th1) cells into inflamed tissues. SEL-E acts as a receptor for cluster of differentiation 44 (CD44 (HCELL)), Mac-2 binding protein, mucin 1 (Muc-1), death receptor-3 and E-selectin ligand-1 (ESL-1). Overexpression of the gene might affect adhesion and migration of the cells leading to the endometriosis disorder.
Physical form
Solution, 0.2 μm filtered, in 0.01 M phosphate buffered saline, pH 7.4.
Preparation Note
For continuous use, store sterile at 2-8°C. For extended storage freeze in sterile working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |
|---|---|
| description clone 1.2B6, purified from hybridoma cell culture | description for serum, plasma, cell culture supernatants and urine |
| Gene Information human ... SELE(6401) | Gene Information human ... SELE(6401) |
| conjugate unconjugated | conjugate - |
| clone 1.2B6, monoclonal | clone - |
| biological source mouse | biological source - |
| antibody form purified immunoglobulin | antibody form - |
| species reactivity human, pig | species reactivity human |
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Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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