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Anti-E-Selectin (CD62E) antibody, Mouse monoclonal

Name: Anti-E-Selectin (CD62E) antibody, Mouse monoclonal
Brand: SIGMA
Price: 479.4 USD
Availability: InStock

clone 1.2B6, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-CD62E, Anti-E-Selectin, Anti-ELAM-1

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$564.00 $479.40

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

1.2B6, monoclonal

Application:

ELISA (c), ELISA (i), FACS, IF, IHC (f), IP, WB

Citations:

$479.40

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1.2B6, monoclonal

form

buffered aqueous solution

mol wt

antigen 100-115 kDa

species reactivity

human, pig

concentration

~2 mg/mL

technique(s)

capture ELISA: suitable, flow cytometry: suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1-2 μg/mL using using 293-T cells transfected with E-selectin fusion protein, western blot: suitable (non-reduced)

isotype

IgG1

UniProt accession no.

P16581

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SELE(6401)

Description

General description

CD62E (E-Selectin) surface antigen is a glycoprotein and member of the selectin family of adhesion receptors. It is expressed by activated human endothelial cells and is detectable in the plasma.

Monoclonal anti-E-selectin (CD62E) (mouse IgG1 isotype) is derived from the 1.2B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with TNF-α activated human endothelial cells. This antibody reacts specifically with E-selectin (CD62E) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant human P-selectin and human platelet P-selectin. Cross-reactivity has been observed with porcine E-selectin.
It is a 140KDa protein mainly expressed in endothelial cells. E- selectin is characterized with an N-terminal domain, epidermal growth factor (EGF)-like domain, lectin (ligand-binding) domain, variable short consensus repeats and transmembrane domain.

Selectin E (SELE) is encoded by the gene mapped to human chromosome 1q24.2. The encoded protein is mainly expressed on luminal surfaces of inflamed vasculature.

Immunogen

TNF-α activated human endothelial cells.

Application

Anti-E-Selectin (CD62E) antibody, Mouse monoclonal has been used in immunofluorescence.

Biochem/physiol Actions

E-selectin acts as a key inflammatory cytokine. E-selectin-dependent rolling helps in binding pancreatic cancer cell to hyaluronic acid. In addition, the encoded protein in association with P-selectins, plays a vital role in recruiting leukocytes to inflammatory sites. Mutation in the gene is associated with the development of various diseases such as atherosclerosis and stroke.

Reacts specifically with human CD62E (E-Selectin, ELAM-1) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant hman P-Selectin and human platelet P-Selectin. Cross-reactivity has been observed with porcine E-Selectin. Monoclonal Anti-human E-Selctin inhibits adhesion of neutrophils, eosinophils, and a skin-homing T-cell subpopulation to activated endothelial cells and CD62E-transfected cells. 5th Workshop: code no. E 040.

Selectins play a vital role in maintaining homoeostasis and cutaneous inflammation. SEL-E and SEL-P help in carrying T helper (Th1) cells into inflamed tissues. SEL-E acts as a receptor for cluster of differentiation 44 (CD44 (HCELL)), Mac-2 binding protein, mucin 1 (Muc-1), death receptor-3 and E-selectin ligand-1 (ESL-1). Overexpression of the gene might affect adhesion and migration of the cells leading to the endometriosis disorder.

Physical form

Solution, 0.2 μm filtered, in 0.01 M phosphate buffered saline, pH 7.4.

Preparation Note

For continuous use, store sterile at 2-8°C. For extended storage freeze in sterile working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4700591	SAB4700489	SAB4700490
Sigma-Aldrich S9555 Anti-E-Selectin (CD62E) antibody, Mouse monoclonal	Sigma-Aldrich SAB4700591 Monoclonal Anti-CD62L, low endotoxin antibody produced in rat	Sigma-Aldrich SAB4700489 Monoclonal Anti-CD62L antibody produced in mouse	Sigma-Aldrich SAB4700490 Monoclonal Anti-CD62L-APC antibody produced in mouse
description clone 1.2B6, purified from hybridoma cell culture	description clone MEL-14, purified immunoglobulin, buffered aqueous solution	description clone LT-TD180, purified immunoglobulin, buffered aqueous solution	description clone LT-TD180, purified immunoglobulin, buffered aqueous solution
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
biological source mouse	biological source rat	biological source mouse	biological source mouse
Gene Information human ... SELE(6401)	Gene Information mouse ... Sell(20343)	Gene Information human ... SELL(6402)	Gene Information human ... SELL(6402)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate Allophycocyanin conjugate
species reactivity human, pig	species reactivity mouse	species reactivity human	species reactivity human
clone 1.2B6, monoclonal	clone MEL-14, monoclonal	clone LT-TD180, monoclonal	clone LT-TD180, monoclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c