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Superoxide Dismutase from human erythrocytes

Name: Superoxide Dismutase from human erythrocytes
Brand: SIGMA
Price: 225 USD
Availability: InStock

essentially salt-free, lyophilized powder, ≥2,500 units/mg protein

Synonym(s):

SOD, Superoxide: superoxide oxidoreductase

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Size/SKU	Availability	Price
1000 units	Available to ship TODAYfromMILWAUKEE	$225.00
3000 units	Available to ship TODAYfromMILWAUKEE	$658.00
15000 units	Available to ship TODAYfromMILWAUKEE	$2,250.00 $1,912.50
30000 units	Available to ship TODAYfromMILWAUKEE	$3,720.00

About This Item

CAS Number:

9054-89-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-943-0

MDL number:

MFCD00132404

EC Number:

1.15.1.1 (BRENDA, IUBMB)

Specific activity:

≥2,500 units/mg protein

Assay:

>80% protein (biuret)

Biological source:

human erythrocytes

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Properties

biological source

human erythrocytes

Quality Segment

400

assay

>80% protein (biuret)

form

essentially salt-free, lyophilized powder

specific activity

≥2,500 units/mg protein

mol wt

32.0 kDa

composition

Protein, ≥80% biuret

manufacturer/tradename

Sigma-Aldrich

technique(s)

activity assay: suitable

color

white to off-white

pH range

7.6—10.5

pH

7.8

suitability

suitable for molecular biology

UniProt accession no.

P00441, P04179, P08294

application(s)

life science and biopharma

storage temp.

−20°C

Gene Information

human ... SOD1(6647), SOD2(6648), SOD3(6649)

Description

General description

Superoxide dismutases (SOD) are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. Three forms of SOD exist, based on the metal ions in the active site. These are Cu²⁺/Zn²⁺, Mn²⁺ and Fe²⁺ containing SOD. In vertebrate organisms, Cu/Zn-SOD is located in the cytoplasm as well as the mitochondrial intermembrane space, whereas Mn-SOD is located at the mitochondrial matrix space in prokaryotes. Fe-SOD is also found in prokaryotes and higher plants. Human erythrocyte SOD is a non-covalently bound homodimeric protein with two 16.3 kDa subunits containing 153 amino acids. Each dimer consists of two Cu²⁺atoms and two Zn²⁺ atoms.

Application

Superoxide Dismutase from human erythrocytes has been used:

to test its effect on human neutrophils in reactive oxygen species (ROS) measurement studies involving Pseudomonas aeruginosa infection
as an antioxidant to test its effect on ROS generation induced by atmospheric-pressure plasma jet (APPJ) in red blood cell (RBC) homogenates using optical spectroscopy studies
to test its attenuating effect on hemoglobin (Hb)-induced nuclear factor-kappa B (NF- κB) and hypoxia-inducible factor (HIF) activity in human dermal microvascular endothelial cells (HMECs-1)
as a reference antioxidant protein to examine its expression in human intestinal Caco-2 cells following treatment with dietary flavonoids
in combination with catalase to promote cell differentiation in vitro

Biochem/physiol Actions

Mutations in the SOD1 gene are implicated in Amyotrophic lateral sclerosis (ALS).

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Analysis Note

For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).

Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀of 0.025 ± 0.005 per min.

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This Item	S7571	S8160	S8409
Sigma-Aldrich S9636 Superoxide Dismutase from human erythrocytes	Sigma-Aldrich S7571 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S8160 Superoxide Dismutase from bovine liver	Sigma-Aldrich S8409 Superoxide Dismutase from bovine liver
description essentially salt-free, lyophilized powder, ≥2,500 units/mg protein	description lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret	description lyophilized powder, ≥1500 units/mg protein	description ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)
specific activity ≥2,500 units/mg protein	specific activity ≥3,000 units/mg protein	specific activity ≥1500 units/mg protein	specific activity 2,000-6,000 units/mg protein (biuret)
technique(s) activity assay: suitable	technique(s) immunoblotting: suitable, inhibition assay: suitable	technique(s) -	technique(s) -
biological source human erythrocytes	biological source bovine	biological source -	biological source -
Gene Information human ... SOD1(6647), SOD2(6648), SOD3(6649)	Gene Information cow ... SOD1(281495), SOD2(281496)	Gene Information cow ... SOD1(281495)	Gene Information cow ... SOD1(281495)
assay >80% protein (biuret)	assay -	assay -	assay -
suitability suitable for molecular biology	suitability -	suitability -	suitability -

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c