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Superoxide Dismutase bovine

Name: Superoxide Dismutase bovine
Brand: SIGMA
Price: 105 USD
Availability: InStock

recombinant, expressed in E. coli, lyophilized powder, ≥2500 units/mg protein, ≥90% (SDS-PAGE)

Synonym(s):

Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, CU/ZN-SOD, SOD, SOD1, Superoxide: superoxide oxidoreductase

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Size/SKU	Availability	Price
15000 units	Check Cart for Availability	$105.00
30000 units	Check Cart for Availability	$153.00
75000 units	Estimated to ship onOctober 15, 2026fromMILWAUKEE	$307.00
300000 units	Check Cart for Availability	$1,150.00 $977.50

About This Item

CAS Number:

9054-89-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-943-0

MDL number:

MFCD00132404

EC Number:

1.15.1.1 (BRENDA, IUBMB)

Specific activity:

≥2500 units/mg protein

Assay:

≥90% (SDS-PAGE)

Biological source:

bovine

Recombinant:

expressed in E. coli

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Properties

biological source

bovine

Quality Segment

200

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥2500 units/mg protein

storage condition

(Tightly closed)

technique(s)

inhibition assay: suitable

color

white

optimum pH

7.8 (25 °C)

pH range

7.6-10.5

pI

4.95

sequence note

MATKAVCVLKGDGPVQGTIHFEAKGDTVVVTGSITGLTEGDHGFHVHQFGDNTQGCTSAGPHFNPLSKKHGGPKDEERHVGDLGNVTADKNGVAIVDIVDPLISLSGEYSIIGRTMVVHEKPDDLGRGGNEESTKTGNAGSRLACGVIGIAK

NCBI accession no.

NM_174615, NP_777040

UniProt accession no.

P00442

storage temp.

−20°C

Description

General description

Research area: Cell Signaling

SOD from bovine erythrocytes was the first SOD to be found in mammalian tissues. There are three forms of SOD differentiated by the metal ions in the active site. These are Cu⁺²/Zn⁺², Mn⁺², and Fe⁺²SOD. In vertebrates, Cu/Zn-SOD is found in the cytoplasm, chloroplast, and may be in extracellular space, while Mn-SOD is found in the mitochondrial matrix space and peroxisome. Fe-SOD is found in the chloroplast of prokaryotes and some higher plants.

Application

Superoxide Dismutase bovine has been used:

to construct a calibration curve for the evaluation of superoxide dismutase (SOD) enzyme activities
in a study to investigate where lipoproteins may affect the L-arginine-nitric oxide pathway
in a study to investigate the mass spectral evidence for carbonate-anion-radical-induced posttranslational modification of tryptophan to kynurenine in human Cu, Zn superoxide dismutase

Biochem/physiol Actions

Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. This reaction in turn activates redox-sensitive kinases and inactivates specific phosphatases to regulate redox-sensitive signaling pathway, including hypertrophy, proliferation, and migration. SOD serves as a potent antioxidant and protects the cells against the toxic effects of oxygen radicals. SOD may also suppress apoptosis by competing with nitric oxide (NO) for superoxide anion, which reacts with NO to form peroxynitrite, an inducer of apoptosis.

Preparation Note

Produced using animal component-free materials.

Reconstitute in 10 mM potassium phosphate, pH 7.4.

Analysis Note

Extinction coefficient: E^mM= 10.3 (258 nM)
SOD has no significant absorbance peak at 280 nM because of the absence of tryptophan.

Other Notes

Inhibitors: cyanide, OH^- (competitive), hydrogen peroxide

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

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This Item	S8160	S7571	S5395
Sigma-Aldrich S9697 Superoxide Dismutase bovine	Sigma-Aldrich S8160 Superoxide Dismutase from bovine liver	Sigma-Aldrich S7571 Superoxide Dismutase from bovine erythrocytes	Sigma-Aldrich S5395 Superoxide Dismutase from bovine erythrocytes
description recombinant, expressed in E. coli, lyophilized powder, ≥2500 units/mg protein, ≥90% (SDS-PAGE)	description lyophilized powder, ≥1500 units/mg protein	description lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret	description BioReagent, ≥3,000 units/mg protein, suitable for cell culture, lyophilized powder
technique(s) inhibition assay: suitable	technique(s) -	technique(s) immunoblotting: suitable, inhibition assay: suitable	technique(s) cell culture \| mammalian: suitable
biological source bovine	biological source -	biological source bovine	biological source bovine erythrocytes
specific activity ≥2500 units/mg protein	specific activity ≥1500 units/mg protein	specific activity ≥3,000 units/mg protein	specific activity ≥3,000 units/mg protein
assay ≥90% (SDS-PAGE)	assay -	assay -	assay -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant -

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c