The isotype of this product, Anti-IDH1 (R132H) antibody, Mouse monoclonal, is IgG. It has not been further determined if it is IgG1 or IgG2, or if it has a lambda or kappa chain.
SAB4200548
Anti-IDH1 (R132H) antibody, Mouse monoclonal
clone HMab-1, purified from hybridoma cell culture
Synonym(s):
Anti-IDCD, Anti-IDH, Anti-IDP, Anti-IDPC, Anti-Isocitrate dehydrogenase 1 (NADP+), soluble, Anti-NADP(+)-specific ICDH, Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Anti-NADP-dependent isocitrate dehydrogenase, peroxisomal, Anti-Oxalosuccinate decarboxylase, Anti-PICD
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| Size/SKU | Availability | Price |
|---|---|---|
200 μL | Check Cart for Availability | $641.00 |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
HMab-1, monoclonal
Application:
WB
Citations:
14
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HMab-1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
western blot: 4.0-8.0 μg/mL using extract of HEK-293T cells overexpressing IDH1R132H.
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IDH1(3417)
General description
Anti-IDH1 (R132H) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma HMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to mutation R132H of human IDH1. A member of isocitrate dehydrogenase (IDH) family, IDH1, is the human cytoplasmic NADP-specific enzyme. Its subcellular localization was shown to be in both peroxisomes and the cytoplasm.
Immunogen
peptide corresponding to mutation R132H of human IDH1.
Application
Anti-IDH1 (R132H) antibody has been used:
- in immunoblotting
- in immunohistochemistry
- in western blotting
- in cell cycle analysis and apoptosis assays
- in chromatin immunoprecipitation (ChIP) assay
- in in vitro migration assay
Biochem/physiol Actions
Isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate into α α -KG) using NADP as co-substrate. Mutations in IDH1 are specific to Arg132 (R132) and endow them with the function of generating 2-hydroxyglutarate (2HG) instead of α-KG. This product alters gene transcription through effects on DNA and histone methylation. Several IDH1 mutations exist, including R132H, R132C, R132S, R132G and R132L. Each may result in different tumor type with varied malignant progression. The most frequent known mutation (>90%) is the alteration of arginine to histidine (R132H). Hence, antibodies that recognize the IDH1R132H mutation can be useful for the diagnosis of mutation-bearing tumors like gliomas.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description clone HMab-1, purified from hybridoma cell culture | description clone HMab-1, from mouse | description affinity isolated antibody | description affinity isolated antibody, buffered aqueous solution |
| biological source mouse | biological source mouse | biological source rabbit | biological source rabbit |
| clone HMab-1, monoclonal | clone HMab-1, monoclonal | clone polyclonal | clone polyclonal |
| Gene Information human ... IDH1(3417) | Gene Information human ... IDH1(3417) | Gene Information human ... IDH1(3417) | Gene Information human ... IDH1(3417) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| species reactivity human | species reactivity human | species reactivity guinea pig, horse, rabbit, sheep, rat, human, mouse, bovine, dog | species reactivity human, rat, mouse |
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Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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