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Anti-β-Catenin antibody, Mouse monoclonal

Name: Anti-β-Catenin antibody, Mouse monoclonal
Brand: SIGMA
Price: 507 USD
Availability: InStock

clone 15B8, purified from hybridoma cell culture

Synonym(s):

Anti-CTNNB1, Anti-armadillo

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$507.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

15B8, monoclonal

Application:

IF, IHC, IP

Citations:

$507.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

15B8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~94 kDa

species reactivity

chicken, mouse, human, rat, monkey, hamster, bovine, canine

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract, immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

P35222

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Description

General description

Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily.

Immunogen

recombinant chicken β-Catenin

Application

Anti-β-Catenin antibody, Mouse monoclonal has been used in:

immunoblotting
immunofluorescence
immunohistochemistry
immunoprecipitation

Biochem/physiol Actions

β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7738	SAB4200715	SAB4500545
Sigma-Aldrich SAB4200720 Anti-β-Catenin antibody, Mouse monoclonal	Sigma-Aldrich C7738 Anti-β-Catenin−Cy3 antibody, Mouse monoclonal	Sigma-Aldrich SAB4200715 Anti-β-tubulin antibody, Mouse monoclonal	Sigma-Aldrich SAB4500545 Anti-Catenin-β antibody produced in rabbit
description clone 15B8, purified from hybridoma cell culture	description clone 15B8, purified from hybridoma cell culture	description clone 2-28-33, purified from hybridoma cell culture	description affinity isolated antibody
species reactivity chicken, mouse, human, rat, monkey, hamster, bovine, canine	species reactivity chicken, bovine, human, canine	species reactivity dog, chicken, rat, bovine, monkey, human, mouse	species reactivity rat, mouse, human
conjugate unconjugated	conjugate CY3 conjugate	conjugate unconjugated	conjugate unconjugated
antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody
clone 15B8, monoclonal	clone 15B8, monoclonal	clone 2-28-33, monoclonal	clone polyclonal
Gene Information human ... CTNNB1(1499) mouse ... Ctnnb1(12387) rat ... Ctnnb1(84353)	Gene Information human ... CTNNB1(1499)	Gene Information human ... TUBB(203068) mouse ... Tubb3(22152)	Gene Information human ... CTNNB1(1499)
biological source mouse	biological source mouse	biological source mouse	biological source rabbit

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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