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Anti-Vinculin Antibody

Name: Anti-Vinculin Antibody
Brand: SIGMA
Price: 581 USD
Availability: InStock

mouse monoclonal, VIN-11-5

Synonym(s):

Anti-MV, Anti-Metavinculin, Anti-VINC1

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$581.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

VIN-11-5, monoclonal

Species reactivity:

human, monkey, rat, mouse, guinea pig, bovine, hamster, chicken, dog

Application:

IF, IHC, IP

Citations:

$581.00

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Properties

Product Name

Anti-Vinculin antibody, Mouse monoclonal, clone VIN-11-5, purified from hybridoma cell culture

biological source

mouse

Quality Segment

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

VIN-11-5, monoclonal

form

buffered aqueous solution

mol wt

~120 kDa

species reactivity

human, monkey, rat, mouse, guinea pig, bovine, hamster, chicken, dog

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.125-0.25 μg/mL using mouse myoblast C2C12 cell extract, immunofluorescence: 5-10 μg/mL, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

P18206

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Vcl(22330)

Description

General description

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the VIN-11-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with smooth muscle vinculin from chicken gizzard. Vinculin, also known as metavinculin is highly conserved scaffolding protein localized to focal adhesions and adherens junctions. Vinculin is a 116 kDa protein with 1066 aminoacids. Vinculin comprises eight anti-parallel α-helical bundles organized into five distinct domains. Domains 1-3 are arranged into a tri-lobar head with a molecular mass of 95 kDa and diameter of 80Å . The vinculin head binds many proteins including talin, IpaA, β-catenin, α-catenin and α-actinin. A 61 amino acid, proline-rich linker region (residues 837-878) connects the head and tail. The linker binds vasodilator-stimulated phosphoprotein (VASP), vinexin, ponsin and Arp2/3. The vinculin tail (amino acids 879-1066) is a 30 kDa helical bundle containing five helices connected by short loops (3-8 residues). The tail contains binding sites for vinculin head domain, paxillin, acidic phospholipids and actin. Within the cell vinculin exists either as open, active form or as a closed, auto-inhibited state. The vinculin gene is mapped to human chromosome 10q22.2. Metavinculin (150 kDa), a splice variant of vinculin, is co-expressed with vinculin in muscle tissues. These proteins are localized to the cell membrane, the I-band in the sarcomere and to the intercalated discs.

Immunogen

smooth muscle vinculin from chicken gizzard

Application

Monoclonal Anti-Vinculin is recommended to use in various immunochemical assays, including immunoblot (~120kDa), immunofluorescence, immunohistochemistry and immunoprecipitation.

Biochem/physiol Actions

Vinculin is a cytoplasmic actin binding protein. It plays a key role in cell to cell and cell to matrix adhesion. It mediates adhesion by directly binding to F-actin and subsequently causing actin polymerization and binding of actin remodelling proteins. At high concentrations, Vinculin can undergo oligomerization and may be involved in the transmembrane assembly of adhesion plaques. Vinculin has been implicated in the control of adhesion, cell morphology and motility and muscle functions. Mutations in vinculin, affects the binding of vinculin to F-actin and impairs cell migration. It is a susceptible gene for dilated and hypertrophic cardiomyopathy. Mutations in vinculin may lead to sudden unexplained nocturnal death syndrome (SUNDS). In relation to cell adhesion and motility function, Vinculin is considered to have a crucial effect on tumor cells ability to invade tissues and hence to metastasize. Thus, it is suggested to be implicated in diagnosis of cancer progression and metastasis prognosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	V9264	V4505	SAB4200080
Sigma-Aldrich SAB4200729 Anti-Vinculin antibody, Mouse monoclonal	Sigma-Aldrich V9264 Anti-Vinculin antibody, Mouse monoclonal	Sigma-Aldrich V4505 Monoclonal Anti-Vinculin antibody produced in mouse	Sigma-Aldrich SAB4200080 Anti-Vinculin antibody, Mouse monoclonal
description clone VIN-11-5, purified from hybridoma cell culture	description clone hVIN-1, purified from hybridoma cell culture	description clone VIN-11-5, ascites fluid	description clone V284, purified from hybridoma cell culture
antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form ascites fluid	antibody form purified immunoglobulin, purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity human, monkey, rat, mouse, guinea pig, bovine, hamster, chicken, dog	species reactivity frog, chicken, mouse, canine, human, bovine, rat, turkey	species reactivity bovine, human, chicken, mouse	species reactivity rabbit, chicken, human, rat, monkey, mouse, canine
Gene Information mouse ... Vcl(22330)	Gene Information human ... VCL(7414) mouse ... Vcl(22330) rat ... Vcl(305679)	Gene Information human ... VCL(7414) mouse ... Vcl(22330)	Gene Information human ... VCL(7414) mouse ... Vcl(22330) rat ... Vcl(305679)
clone VIN-11-5, monoclonal	clone hVIN-1, monoclonal	clone VIN-11-5, monoclonal	clone V284, monoclonal
form buffered aqueous solution	form buffered aqueous solution	form -	form -

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

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