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Anti-Protein A antibody, Mouse Monoclonal

Name: Anti-Protein A antibody, Mouse Monoclonal
Brand: SIGMA
Price: 534 USD
Availability: InStock

clone SPA-27, purified from hybridoma cell culture

Synonym(s):

Anti-IgG binding protein A, Anti-SpA, Anti-Staphylococcal protein A, Anti-Staphylococcus ayreus protein A

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$534.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Clone:

SPA-27, monoclonal

Species reactivity:

Staphylococcus aureus

Application:

DB, ELISA (i), FACS, IF, IP

Citations:

$534.00

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Properties

biological source

mouse

Quality Segment

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SPA-27, monoclonal

form

buffered aqueous solution

mol wt

~42 kDa

species reactivity

Staphylococcus aureus

concentration

~1.0 mg/mL

technique(s)

dot blot: suitable, flow cytometry: suitable, immunoblotting: suitable, immunofluorescence: suitable, immunoprecipitation (IP): 2.5-5 μg/test using purified protein A from Staphylococcus aureus, indirect ELISA: 0.25-0.5 μg/mL using 10 μg/mL of protein A from Staphylococcus aureus for coating. To reduce nonspecific binding we recommend on detection using a secondary anti-mouse, F(ab’)2 fragment antibody

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Staphylococcus aureus protein A, also known as SpA or IgG binding protein A, is a 42 kDa single chain polypeptide isolated from the cell wall of S. aureus Cowan I strain. It consists of a single polypeptide chain containing five repetitive immunoglobulin-binding domains, rich in aspartic and glutamic acids.Anti-Protein A antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SPA-27 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse.

Immunogen

Protein A from Staphylococcus aureus Cowan I strain

Application

The antibody may be used in various immunochemical techniques including immunoblotting, dot blot, ELISA, immunofluorescence, immunoprecipitation and flow cytometry (FACS).

Biochem/physiol Actions

Monoclonal Anti-Protein A specifically recognizes Protein A from Staphylococcus aureus bacteria.

Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used either in its native form, conjugated to various markers or serve as a tag for a specific protein of interest. In addition, protein A has low affinity to mouse monoclonal antibodies from IgG1 isotype. Thus, a monoclonal anti-protein A antibody of the mouse IgG1 isotype has an advantage due to its specific targeting and binding to protein A via its Fab fragment. Monoclonal anti-protein A antibody can serve a useful tool for quantification and visualization of S. aureus bacteria and bacterial contaminations in different examined samples and for detection of protein A tagged proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the material safety data sheet (MSDS) for information regarding hazards and safe handling practices.

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This Item	P2921	SAB4200552	T1076
Sigma-Aldrich SAB4200745 Anti-Protein A antibody, Mouse Monoclonal	Sigma-Aldrich P2921 Monoclonal Anti-Protein A antibody produced in mouse	Sigma-Aldrich SAB4200552 Anti-WASH1 antibody, Mouse monoclonal	Sigma-Aldrich T1076 Anti-TAP antibody, Mouse monoclonal
description clone SPA-27, purified from hybridoma cell culture	description clone SPA-27, ascites fluid	description clone WASH1-27, purified from hybridoma cell culture	description clone 53H8, purified from hybridoma cell culture
clone SPA-27, monoclonal	clone SPA-27, monoclonal	clone WASH1-27, monoclonal	clone 53H8, monoclonal
species reactivity Staphylococcus aureus	species reactivity -	species reactivity mouse, human	species reactivity human
antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
form buffered aqueous solution	form -	form buffered aqueous solution	form buffered aqueous solution
technique(s) indirect ELISA: 0.25-0.5 μg/mL using 10 μg/mL of protein A from Staphylococcus aureus for coating. To reduce nonspecific binding we recommend on detection using a secondary anti-mouse, F(ab’)2 fragment antibody, immunoprecipitation (IP): 2.5-5 μg/test using purified protein A from Staphylococcus aureus, immunofluorescence: suitable, dot blot: suitable, flow cytometry: suitable, immunoblotting: suitable	technique(s) indirect ELISA: 1:20,000, western blot: suitable, immunocytochemistry: suitable	technique(s) immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells, western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells overexpressing mouse WASH1	technique(s) indirect ELISA: suitable, immunoprecipitation (IP): suitable, western blot: 0.5-1 μg/mL using HeLa nuclear cell extract, immunocytochemistry: suitable, microarray: suitable

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

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