This product is IgG1 isotype from mouse. Please see the link below to review further details are available on the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/665/651/sab4200751dat.pdf
SAB4200751
Anti-CRISPR/Cas9 (C-terminal) antibody, Mouse monoclonal
clone 10C11-A12, purified from hybridoma cell culture
Synonym(s):
Anti-CRISPR-associated protein-9 nuclease, Anti-Crispr, Anti-Crsipr RNA
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| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Available to ship TODAYfromMILWAUKEE | $579.00 |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
10C11-A12, monoclonal
Application:
IF, IP
Citations:
5
conjugate
unconjugated
Quality Segment
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
10C11-A12, monoclonal
form
buffered aqueous solution
concentration
~1.00 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using whole extracts of human HEK-293T cells over-expressing CAS9 protein, immunofluorescence: 1.25-2.5 μg/mL using human HEK-293T cells over-expressing CAS9 protein, immunoprecipitation (IP): 5-10 μg/test using whole extract of human HEK-293T cells over-expressing CAS9 protein
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
CAS9, also known as clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein-9 nuclease, is the signature gene of the type II CRISPR -RuvC (RNase H-like fold) Cas system. CAS9 contains 1388 amino acids. This protein is predicted to contain a RuvC/ ribonuclease (RNase) H domain involved in CRISP RNA (crRNA) maturation and McrA/HNH signature domain involved in the DNA degradation step.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) belongs to the type II CRISPR/CAS9 system. It is part of an adaptive immune system of the Streptococcus pyogenes SF370, protecting from pathogens′ target genes by cleaving the foreign DNA in a sequence-dependent manner. The type II CRISPR/Cas system which has been adapted to expression in eukaryotic cells, consists of four genes including the Cas9 (CRISPR-associated proteins) nuclease, two noncoding CRISPR RNAs (crRNAs, or gRNA), trans-activating crRNA (tracrRNA) and a precursor crRNA (pre-crRNA) array. The pre-crRNA contains nuclease guide sequences (spacers) interspaced by identical direct repeats (DRs).
The Cas9 endonuclease can be engineered with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
In comparison to other genome-editing technologies such as designer zinc fingers (ZFs), transcription activator–like effectors (TALEs) and homing meganucleases, the CRISPR/CAS9 system is a scalable, affordable and easy to engineer. Therefore, the anti-CRISPR/CAS9 antibody can be a useful tool for detecting CRISPR/CAS9 positively transfected cells, revealing DSB sites in the genome and in ChIP (Chromatin Immunoprecipitation) related assays
The Cas9 endonuclease can be engineered with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
In comparison to other genome-editing technologies such as designer zinc fingers (ZFs), transcription activator–like effectors (TALEs) and homing meganucleases, the CRISPR/CAS9 system is a scalable, affordable and easy to engineer. Therefore, the anti-CRISPR/CAS9 antibody can be a useful tool for detecting CRISPR/CAS9 positively transfected cells, revealing DSB sites in the genome and in ChIP (Chromatin Immunoprecipitation) related assays
Immunogen
Recombinant protein within the C-terminal region of Streptococcus pyogenes Cas9
Application
Monoclonal Anti-CRISPR/CAS9 (C-terminal) recognizes CAS9 protein in CAS9 construct over-expression systems. The antibody successfully recognizes mutant Cas9 variants, including nickase Cas9 and dead Cas9 (dCas9). The antibody may be used in various immunochemical techniques including Immunoblotting, Immunofluorescence and Immunoprecipitation. Monoclonal Anti- CRISPR/CAS9 (C-terminal) does not cross react with FnCas9 from Francisella novicida bacteria and Cpf1 proteins from Acidaminococcus sp. (strain BV3L6) and Lachnospiraceae bacterium ND2006.
Biochem/physiol Actions
CAS9 plays a vital role in plasmid DNA interference. It is the only Cas protein needed to deliver resistance against foreign DNA. CAS9 stimulates both RNA-guided genome editing and gene regulation in various organisms, but it can facilitate only one activity at a time within any given cell.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
1 of 1
This Item | |||
|---|---|---|---|
| description clone 10C11-A12, purified from hybridoma cell culture | description clone 7A9-3A3, purified from hybridoma cell culture | description recombinant, expressed in HEK 293 cells | description - |
| conjugate unconjugated | conjugate FITC conjugate | conjugate unconjugated | conjugate unconjugated |
| clone 10C11-A12, monoclonal | clone 7A9-3A3, monoclonal | clone 1C12-H1, recombinant monoclonal | clone 8G4, monoclonal |
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified antibody | antibody form purified antibody |
| technique(s) immunofluorescence: 1.25-2.5 μg/mL using human HEK-293T cells over-expressing CAS9 protein, immunoblotting: 1-2 μg/mL using whole extracts of human HEK-293T cells over-expressing CAS9 protein, immunoprecipitation (IP): 5-10 μg/test using whole extract of human HEK-293T cells over-expressing CAS9 protein | technique(s) immunofluorescence: 1:200-1:400 using human HEK-293T cells over-expressing CAS9 protein | technique(s) flow cytometry: suitable, western blot: suitable, immunocytochemistry: suitable | technique(s) immunofluorescence: suitable, western blot: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hello! I would like to know what is the isotype of the Anti-CRISPR/Cas9 (C-terminal) antibody, Mouse monoclonal (SAB4200751). Thanks in advance!
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