Anti-Human IgM (μ chain), CF^™647 antibody produced in goat

CF647 goat anti—human IgM (μ chain) is labeled with our bright, deep red-fluorescent CF647 dye and is prepared from antibodies purified by human IgM—affinity chromatography. The antibodies react specifically with IgM heavy chains (μ chains) and not with immunoglobulin light chains. To minimize cross-reactivity, it has been adsorbed human IgG prior to conjugation

During the B-cell development, IgM (immunoglobulin M) is the first expressed antibody present as a monomer on the surface of naive B cells. Antigenic stimulation and upon maturation, the monomeric IgM units (190kDa), join together to form a pentameric immunoglobulin structure. It also but rarely forms a hexameric structure. The pentameric IgM is linked by a disulfide bond, a polypeptide chain and a J-chain. The μ-chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4.

Human IgM(H+L)

Binds human IgM heavy chain (mu chain) (minimal cross-reactivity IgG and IgA heavy chains, or immuglobulin light chains).

The J-chain of the multimeric IgM (immunoglobulin M) structure is responsible for secretion at the mucosal surfaces. The pentameric form of IgM, has a greater affinity towards antigens (with multiple repeating epitopes) compared to the monomers. The pentameric IgM enables better opsonization of antigens for destruction and fixing complement. IgM is essential of the activation of classical complement system. Reduced serum IgM levels is observed in Bruton′s agammaglobulinemia an X-linked disorder and transient hypogammaglobulinemia in infants.

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Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Protect from light. The antibody solution should be gently mixed before use.

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.