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SAE0049

L-Lactate Dehydrogenase (LDHA)

from human, recombinant, expressed in E. coli, aqueous solution

Synonym(s):

Lactic Dehydrogenase, recombinant from E. coli, α-HBDH, α-hydroxy butyratede hydrogenase, anaerobic lactate dehydrogenase, (S)-Lactate: NAD+ oxidoreductase, L-Lactate Dehydrogenase, Lactate

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Size/SKUAvailabilityPrice
10 kU
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$472.00

About This Item

CAS Number:
9001-60-9
UNSPSC Code:
12352200
NACRES:
NA.54
EC Number:
1.1.1.27 (BRENDA, IUBMB)
MDL number:
MFCD00131466
Biological source:
human
Recombinant:
expressed in E. coli

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biological source

human

Quality Segment

recombinant

expressed in E. coli

form

aqueous solution

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

color

colorless

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LDHA(3939)

General description

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15. It is a subunit of lactate dehydrogenase.In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

Application

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay. It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.

Biochem/physiol Actions

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.
L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism, LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway. LDHA aids in the NAD+ regeneration during the β-oxidation of fatty acid. LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.

Physical form

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

Other Notes

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

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SAB570180759747L7525
L-Lactate Dehydrogenase (LDHA) from human, recombinant, expressed in E. coli, aqueous solution

Sigma-Aldrich

SAE0049

L-Lactate Dehydrogenase (LDHA)

Anti-Phospho-LDHA-Y10 antibody produced in rabbit

Sigma-Aldrich

SAB5701807

Anti-Phospho-LDHA-Y10 antibody produced in rabbit

Sigma-Aldrich

Sigma-Aldrich

59747

Lactic Dehydrogenase, recombinant from E. coli

L-Lactic Dehydrogenase from porcine heart ammonium sulfate suspension, ≥200 units/mg protein

Sigma-Aldrich

L7525

L-Lactic Dehydrogenase from porcine heart

description

from human, recombinant, expressed in E. coli, aqueous solution

description

-

description

≥90 U/mg

description

ammonium sulfate suspension, ≥200 units/mg protein

Gene Information

human ... LDHA(3939)

Gene Information

human ... LDHA(3939)

Gene Information

-

Gene Information

pig ... LDHA(407245)

biological source

human

biological source

rabbit

biological source

-

biological source

Porcine heart

form

aqueous solution

form

liquid

form

powder

form

ammonium sulfate suspension

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

recombinant

-

UniProt accession no.

P00338

UniProt accession no.

P00338

UniProt accession no.

-

UniProt accession no.

P00339

shipped in

dry ice

shipped in

wet ice

shipped in

-

shipped in

-

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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