The reconstituted enzyme stock solution may be stored at -20 °C, in aliquots, for at least several months. The long-term stability of stored stock solutions has not been determined. Avoid repeated freeze–thaw cycles. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/822/sae0158pis-ms.pdf
SAE0158
Chitinase from Streptomyces griseus
chromatographically purified, lyophilized powder, free of DNA contaminants, suitable for Microbiome research
Synonym(s):
Chitinase from Streptomyces griseus, (1→4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase), Chitodextrinase, Hydrolitic enzyme, Lytic Enzyme, Poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase, Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase, beta-1,4-poly-N-acetyl glucosamidinase, poly-beta-glucosaminidase
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| Size/SKU | Availability | Price |
|---|---|---|
10 units | Check Cart for Availability | $204.00 |
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
>200 USP units/mg solid
$204.00
Check Cart for Availability
Quality Segment
specific activity
>200 USP units/mg solid
feature
DNA free
shipped in
dry ice
storage temp.
−20°C
General description
Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeletal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates). Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source. Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
- The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
- The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.
Application
- Agriculture fields: control pathogens.
- Human health care: Asthma.
- Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
- Preparation of single-cell protein
- Isolation of protoplasts from fungi and yeast
- Control of pathogenic fungi
- Treatment of chitinous waste, mosquito control and morphogenesis
Features and Benefits
This Chitinase is free of DNA contaminates, suitable for microbiome research. Cell walls digestion is performed safely by chitin degradation in fungi and other organisms.
Other Notes
One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2-hour assay.
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This Item | |||
|---|---|---|---|
| description chromatographically purified, lyophilized powder, free of DNA contaminants, suitable for Microbiome research | description lyophilized powder (essentially salt free), ≥200 units/g solid | description lyophilized powder, ≥600 units/g solid | description aqueous solution |
| specific activity >200 USP units/mg solid | specific activity ≥200 units/g solid | specific activity ≥600 units/g solid | specific activity - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in - | shipped in wet ice | shipped in dry ice |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| feature DNA free | feature - | feature - | feature - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
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