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SBR00030

Short Chain Fatty Acid Kit

Synonym(s):

Sodium acetate, Sodium propionate, and Sodium butyrate

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Size/SKUAvailabilityPrice
1 kit

Estimated to ship onDecember 11, 2026fromMILWAUKEE

$541.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12352211
eCl@ss:
32160703

$541.00


Estimated to ship onDecember 11, 2026Details


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packaging

pkg of 1 kit

Quality Segment

lipid type

saturated FAs

storage temp.

−20°C

General description

Short chain fatty acids (SCFAs) are saturated fatty acids with less than 6 carbons. SCFAs are produced in the gut by microbial fermentation of dietary fibers.(1) The most important and researched SCFAs are Acetate (Ac), propionate (Pr) and butyrate (Bu).(2) SCFAs are predominantly found in the colon and cross the intestinal epithelium to the blood stream. SCFAs were found to be beneficial and effective in prevention of obesity, Non-alcoholic fatty liver disease and insulin resistance.(3,4)

Application

SCFAs can serve in various applications including in vitro and in vivo administration to study the effect on immune system activation(5), protect epithelial cells from LPS penetration by tight junction formation induction(6), effect insulin secretion(7) and more. In these studies, SCFAs were administrated in vitro at the following concentration range Acetate 1-10mM, propionate and butyrate 0.1-1mM and in vivo Acetate, 70 mM; propionate, 30 mM; butyrate, 20 mM.
Short-chain fatty acids (SCFAs) have diverse roles in various metabolic processes, and their measurement through metabolomics can provide valuable insights into several applications.

Biochem/physiol Actions

Role
  • Regulate metabolism in the gut
  • Indicators of gut health and inflammation
  • Associated with gastrointestinal diseases such as IBS and other conditions such as obesity, colonic carcinogenesis, insulin sensitivity and ischemic stroke

Features and Benefits

  • High-quality chemical, suitable for mulitple research applications
  • Commonly employed in Metabolomics and Biochemical studies

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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This Item
SBR00034SBR00035MAK597
description

-

description

Suitable for mass spectrometry

description

Suitable for mass spectrometry

description

Suitable for 4 x 6-well assays or 16 x 24-well assays

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-10 to -25°C

lipid type

saturated FAs

lipid type

saturated FAs

lipid type

saturated FAs

lipid type

-

packaging

pkg of 1 kit

packaging

-

packaging

-

packaging

-


Storage Class

10 - Combustible liquids



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Questions

1–8 of 8 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Using this kit how many times I can perform the study

    1 answer
    1. This kit contains the following components: 100 mL of 1M Sodium acetate, 100 mL of 0.5 M Sodium propionate, 100 mL of 0.5 M Sodium butyrate, and 1 mL of a short chain fatty acid mix composed of 0.1 M Sodium Acetate, Sodium Propionate, and Sodium Butyrate. Derivation reaction mixtures for detection by LC-MS/HPLC can be prepared as discussed in Table 1 of the technical bulletin.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Sodium acetate: 100 mL/0.1mL per derivation = 1000 derivations
      Sodium propionate: 100 mL/0.2 mL per derivation = 500 derivations
      Sodium Butyrate: 100 mL/0.2 mL per derivation =500 derivations
      Short Chain Fatty Acid Mix: 1 mL/0.1 mL per derivation = 10 derivations

      Please be aware the procedure provides instructions for HPLC, LC-MS and GC-MS analysis. Sample preparation will vary based upon type of analysis.

      Helpful?

  4. Hello. I purchased this product, but it is not listed in the supplementary material, so I am contacting you. What is the solvent that dissolved these SCFAs? Thank you.

    1 answer
    1. The kit components are ready-made aqueous solutions. The concentration of each component included in this kit is as follows:
      1.0 M Sodium Acetate
      0.5 M Sodium Propionate
      0.5 M Sodium Butyrate
      Short Chain Fatty Acid mix – a 0.1 M solution of each above component, Sodium Acetate, Sodium Propionate and Sodium Butyrate, in water.
      Please see the link below to review the product Technical Bulletin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  5. Respected supplier i would like to know what is the application of the kit? Is this a HPLC standard or is it a kit used for detection of butyrate, acetate and propionate? What will i be receiving in this kit? Can you please be more specific?

    1 answer
    1. This kit may be used for a variety of applications, including calibration of downstream detection of SCFAs by HPLC, GC-MS, or LC-MS. The kit components are ready-made aqueous solutions of 1 M Sodium acetate, 0.5 M Sodium propionate, 0.5 M Sodium butyrate, and the Short chain fatty acid mix. Each buffer component has been 0.2 µM filtered and tested for endotoxin. Please see the link below to review additional information, including example analytical data and chromatograms:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  6. mi interesserebbe sapere la concentrazione del prodotto

    1 answer
    1. The concentration and volume of each component included in this kit is as follows:
      1M Sodium Acetate - 100 mL
      0.5M Sodium Propionate - 100 mL
      0.5M Sodium Butyrate - 100 mL
      Short Chain Fatty Acid mix, a 0.1M solution of each above SCFA - 1 mL
      Please see the link below to review the product Technical Bulletin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  7. Is this kit free short chain fatty acids? I do not want the sodium attached.

    1 answer
    1. This product includes individual solutions, as well as a mixture of Sodium Acetate, Sodium Propionate, and Sodium Butyrate. Please review the product datasheet at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  8. Hello is it possible to use this kit for measuring SCFA in fish? please let me know

    1 answer
    1. Unfortunately, there are no guidelines for using this product to measure short-chain fatty acids in a fish sample. Assuming, there is a means to extract the short-chained fatty acids from a fish sample it may be possible to use this kit. Another concern is that the sample will need to be cleaned up with a sample preparation technique before utilizing LC-MS or GC-MS. It may be possible to use this kit for this purpose, but research and method development would be necessary to determine suitability for the intended application.

      Helpful?

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