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SHC012V

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles

Contains a gene encoding TagRFP

Synonym(s):

MISSION®, MISSION® Control Transduction Particles

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1 pkg
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$431.00

About This Item

NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1x106 VP/ml (via p24 assay)
Technique(s):
capture ELISA: 106 TU/mL using p24

$431.00

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Quality Segment

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

General description

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TagRFP Control Transduction Particles are a positive control to monitor transduction efficiency. The MISSION TagRFP Control Transduction Particles are produced from the sequence-verified 8594 base pair lentivirus plasmid, pLKO.1-puro-CMV-TagRFP (SHC012).

This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest.
MISSION®pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used in generating fluorescent cell lines by transduction. It has also been used in transfection of human dermal fibroblast (HDF), WI-38 and IMR-90 FBs to express tagRFP.
MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used to knockdown transient receptor potential vanilloid 1 receptor (TRPV1)-like receptors. It has also been used to generate fluorescent cell lines.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagRFP is a trademark of Evrogen Co.

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This Item
SHC014VSHC003VSHC002H
MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles Contains a gene encoding TagRFP

Sigma-Aldrich

SHC012V

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Transduction Particles Contains a gene encoding TurboGFP, under the control of the UbC promoter

Sigma-Aldrich

SHC014V

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Transduction Particles

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles Green fluorescent protein marker to monitor transduction efficiency

Sigma-Aldrich

SHC003V

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer Targets no known mammalian genes

Sigma-Aldrich

SHC002H

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer

description

Contains a gene encoding TagRFP

description

Contains a gene encoding TurboGFP, under the control of the UbC promoter

description

Green fluorescent protein marker to monitor transduction efficiency

description

Targets no known mammalian genes

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x109 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 109 TU/mL using p24

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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