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SRP0168

EED human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym(s):

HEED, Rinchik 5, WAIT1, WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development

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50 μg
Usually ships in 2 weeks.
$859.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Assay:
≥90% (SDS-PAGE)
Form:
aqueous solution
Mol wt:
51 kDa

$859.00

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biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

51 kDa

packaging

pkg of 50 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EED(8726)

General description

EED (ectoderm development protein) is the part of the polycomb repressive complex 2 (PRC2) where it interacts with EZH2 (enhancer of zeste homolog 2). The protein has seven WD domains (β-transducin domains). The EED gene is mapped to human chromosome 11q14.
Human Embryonic Ectoderm Development (hEED), also known as WAIT-1, GenBank Accession No. NM_003797, amino acids 2-end with N-terminal FLAG-tag, MW = 51kDa, expressed in Sf9 cells via a baculovirus expression system.

Biochem/physiol Actions

EED (ectoderm development protein) is required for proper EZH2 (enhancer of zeste homolog 2)-mediated methyltransferase activity. It also associates with PRC1 (polycomb repressive complex 1) and helps in bringing PRC1 to H3K27me3 (trimethylated lysine residue at position 27 in the protein histone H3) loci. EED also increases PRC1-mediated H2A (histone 2A) ubiquitin E3 ligase activity. Mutations in the EED gene are associated with overgrowth, intellectual disability and dysmorphic features. Change in the activity of EED is linked with the pathogenesis of myeloid disorders.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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HPA061140HPA060089
EED human recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP0168

EED human

Anti-EED antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

HPA061140

Anti-EED antibody produced in rabbit

Anti-EED antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody

Sigma-Aldrich

HPA060089

Anti-EED antibody produced in rabbit

description

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody

recombinant

expressed in baculovirus infected insect cells

recombinant

-

recombinant

-

assay

≥90% (SDS-PAGE)

assay

-

assay

-

biological source

human

biological source

rabbit

biological source

rabbit

concentration

>0.02 mg/mL

concentration

-

concentration

-

form

aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

UniProt accession no.

O75530

UniProt accession no.

O75530

UniProt accession no.

O75530

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