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SRP0221

PTPIA2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

ICA512, Islet cell antigen 2, PTPRN, Protein tyrosine phosphatase-like N, R-PTP-N

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20 μg
Usually ships in 2 weeks.
$550.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Concentration:
>0.02 mg/mL

$550.00

Usually ships in 2 weeks.
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biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

58.8 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPRN(5798)

General description

PTPRN (protein tyrosine phosphatase, receptor type N), more commonly known as IA-2 (insulinoma-associated protein 2), is a transmembrane protein of the PTP family. It was initially identified as a major autoantigen in type-1 diabetes mellitus. In neuroendocrine cells, it resides in the secretory granules. It is a multi-domain protein with intracellular, transmembrane and extracellular domains, and its intracellular region contains a pseudocatalytic domain. It gets processed multiple times during its life cycle, and its different parts are exposed to different cellular and tissue regions such as nucleus, cytosol, organellar lumen in the secretory pathway, extracellular pancreatic islet space, and membrane compartments. This gene is localized to human chromosome 2q35, and codes for a protein of 979 residues.
Protein tyrosine phosphatase. (PTP-IA2, ICA-512), Catalytic domain, (amino acids 693- 979), (Gene bank accession no. NM_002846), with N-terminal GST tag, MW = 58.8 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Biochem/physiol Actions

PTPRN (protein tyrosine phosphatase, receptor type N) is cleaved at the cytosolic domain during insulin secretion. The cytosolic domain is relocated to nucleus where it promotes insulin gene transcription. This protein plays important role in hormone and neuropeptide secretion. This protein is crucial in controlling the dense core vesicle number in β cells and glucose-induced and basal insulin release. PTPRN null mice show reduced dense core vesicle (DCV) number, abnormal insulin secretion, female infertility, behavioral alteration, and circadian dysregulation.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30°C.

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PTPIA2 Active human recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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Sigma-Aldrich

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Anti-IA-2, clone 76F Antibody clone 76F, from mouse

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Anti-IA-2, clone 76F Antibody

Anti-PTPRN antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

HPA007179

Anti-PTPRN antibody produced in rabbit

description

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

description

clone 8E3, purified immunoglobulin, buffered aqueous solution

description

clone 76F, from mouse

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

biological source

human

biological source

mouse

biological source

mouse

biological source

rabbit

Gene Information

human ... PTPRN(5798)

Gene Information

human ... PTPRN(5798)

Gene Information

human ... PTPRN(5798)

Gene Information

human ... PTPRN(5798)

assay

≥90% (SDS-PAGE)

assay

-

assay

-

assay

-

form

aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous glycerol solution

concentration

>0.02 mg/mL

concentration

-

concentration

-

concentration

-

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

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