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SRP0243

aRAF Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

A-Raf proto-oncogene, PKS2, v-Raf murine sarcoma 3611 viral oncogene homolog

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10 μg
Usually ships in 2 weeks.
$543.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$543.00


Usually ships in 2 weeks.

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biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

63.6 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ARAF(369)

General description

Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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description

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

description

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

description

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

description

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥60% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

-

storage condition

avoid repeated freeze/thaw cycles




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