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SRP0255

eIF4E Active human

N-terminal GST-tag, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):

CBP, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein

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100 μg
Usually ships in 2 weeks.
$785.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
51 kDa

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biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

51 kDa

packaging

pkg of 100 μg

concentration

>0.02 mg/mL

technique(s)

cell based assay: suitable

suitability

suitable for molecular biology

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EIF4E(1977)

General description

EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein. It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.

Application

Suitable substrate for MNK1 kinase.

Biochem/physiol Actions

EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.

Physical form

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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This Item
SAB4500735SAB5300107SAB5701176
eIF4E Active human N-terminal GST-tag, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0255

eIF4E Active human

Anti-EIF4E antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4500735

Anti-EIF4E antibody produced in rabbit

Monoclonal Anti-EIF4E antibody produced in mouse clone 5D11, ascites fluid

Sigma-Aldrich

SAB5300107

Monoclonal Anti-EIF4E antibody produced in mouse

Anti-eIF4E antibody produced in rabbit

Sigma-Aldrich

SAB5701176

Anti-eIF4E antibody produced in rabbit

description

N-terminal GST-tag, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

description

affinity isolated antibody

description

clone 5D11, ascites fluid

description

-

biological source

human

biological source

rabbit

biological source

mouse

biological source

rabbit

assay

≥70% (SDS-PAGE)

assay

-

assay

-

assay

-

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

technique(s)

cell based assay: suitable

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

direct ELISA: 1:10,000, immunohistochemistry: 1:200-1:1,000, indirect immunofluorescence: 1:200-1:1,000, flow cytometry: 1:200-1:400, western blot: 1:500-1:2,000

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

concentration

>0.02 mg/mL

concentration

~1 mg/mL

concentration

-

concentration

0.2 mg/mL

form

aqueous solution

form

buffered aqueous solution

form

-

form

liquid

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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