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SRP0730

PIP5K3 (PIKFYVE), Active

PRECISIO® Kinase, recombinant

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10 μg
Usually ships in 2 weeks.
$562.00

About This Item

NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~110 kDa

$562.00

Usually ships in 2 weeks.
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recombinant

expressed in baculovirus infected Sf9 cells

assay

70-95% (SDS-PAGE)

form

frozen liquid

specific activity

90-115 nmol/min·mg (ADP-Glo)

mol wt

~110 kDa

concentration

0.05-0.1 μg/μL

accession no.

NM_015040

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIP5K3(200576)

Application

Useful for kinase assay.

Biochem/physiol Actions

Recombinant human PIP5K3 (1400-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phospha-tidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns (3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity (1). PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome /lysosome compartment refractory to fusion with autop-hagosomes and multivesicular bodies (2).

Preparation Note

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Other Notes

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

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description

PRECISIO® Kinase, recombinant

description

clone 1D11, purified immunoglobulin, buffered aqueous solution

description

PRECISIO® Kinase, recombinant

description

PRECISIO® Kinase, recombinant

assay

70-95% (SDS-PAGE)

assay

-

assay

70-95% (SDS-PAGE)

assay

70-95% (SDS-PAGE)

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

-

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

concentration

0.05-0.1 μg/μL

concentration

-

concentration

0.1 μg/μL

concentration

0.1 μg/μL

form

frozen liquid

form

buffered aqueous solution

form

frozen liquid

form

frozen liquid

mol wt

~110 kDa

mol wt

antigen ~38.21 kDa

mol wt

~73 kDa

mol wt

~75 kDa

specific activity

90-115 nmol/min·mg (ADP-Glo)

specific activity

-

specific activity

4500-6000 nmol/min·mg (ADP-Glo)

specific activity

40-56 nmol/min·mg (radiometric)

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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