Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

SRP2016

GAL4 [(1-147) - AH] from Saccharomyces cerevisiae

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

Gal81

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
10 μg
Please contact Customer Service for Availability
$470.00

About This Item

NACRES:
NA.26
UNSPSC Code:
12352200
Assay:
≥80% (SDS-PAGE)
Biological source:
Saccharomyces cerevisiae
Recombinant:
expressed in E. coli
Concentration:
300 μg/mL

$470.00

Please contact Customer Service for Availability
​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Saccharomyces cerevisiae

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~18.6 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

300 μg/mL

technique(s)

electrophoretic mobility shift assay: suitable

color

colorless to clear

NCBI accession no.

NM_001184062

UniProt accession no.

P04386

shipped in

dry ice

storage temp.

−70°C

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)

Biochem/physiol Actions

Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
SRP2018SRP2017SRP2068
GAL4 [(1-147) - AH] from Saccharomyces cerevisiae recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP2016

GAL4 [(1-147) - AH] from Saccharomyces cerevisiae

GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP2018

GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus

GAL4 [(1-147) + VP16 (411-490)] from Saccharomyces cerevisiae human herpesvirus 2 recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP2017

GAL4 [(1-147) + VP16 (411-490)] from Saccharomyces cerevisiae human herpesvirus 2

SRC1, His tagged, TEV site, human recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP2068

SRC1, His tagged, TEV site, human

assay

≥80% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

biological source

Saccharomyces cerevisiae

biological source

Saccharomyces cerevisiae, canine

biological source

Saccharomyces cerevisiae, human herpesvirus 2

biological source

human

technique(s)

electrophoretic mobility shift assay: suitable

technique(s)

electrophoretic mobility shift assay: suitable

technique(s)

electrophoretic mobility shift assay: suitable

technique(s)

-

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)
human herpesvirus 2 ... HS2VP16A(1487335)

Gene Information

human ... NCOA1(8648)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

concentration

300 μg/mL

concentration

100 μg/mL

concentration

500 μg/mL

concentration

400 μg/mL

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters