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SRP2017

GAL4 [(1-147) + VP16 (411-490)] from Saccharomyces cerevisiae human herpesvirus 2

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

VP16

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10 μg
Usually ships in 2 weeks.
$486.00

About This Item

NACRES:
NA.26
UNSPSC Code:
12352200
Assay:
≥80% (SDS-PAGE)
Biological source:
Saccharomyces cerevisiae, human herpesvirus 2
Recombinant:
expressed in E. coli
Concentration:
500 μg/mL

$486.00

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biological source

Saccharomyces cerevisiae, human herpesvirus 2

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~27.8 kDa

packaging

pkg of 10 and 500 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

500 μg/mL

technique(s)

electrophoretic mobility shift assay: suitable

color

colorless to clear

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)
human herpesvirus 2 ... HS2VP16A(1487335)

General description

VP16 is a part of the tegument of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) particles, and is a prototypical acidic activator. GAL4-VP16 is constructed by the fusion of the acidic activation domain of the HSV (herpes simplex virus) VP16 transactivator to the DNA binding domain of GAL4. VP16 (411-490) is the 89-residue activation domain of VP16 protein.

Biochem/physiol Actions

The GAL4 yeast protein is responsible for the induction of various genes involved in galactose metabolism. This requires the binding of GAL4 gene to the upstream activation sites containing the consensus sequence 5′-CGGN5(T/A)N5CCG-3′. VP16 protein is essential for inducing the expression of viral immediate-early (IE) genes. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The highly acidic C-terminal tail of VP16 functions as a potent transcriptional activator in mammalian cells. GAL4-VP16 construct functions as an activator which promotes transcription in mammalian cells.
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Herpes virus VP16 activates expression of immediate early genes in virally-infected cells. As most other eukaryotic transcriptional activator proteins, VP16 has a modular domain structure: it′s N-terminus is involved in DNA-protein interactions, while its C-terminal 79 amino acids have proven to be an especially potent transactivation domain. When fused to the DNA-binding domain of the yeast GAL4, this VP16 fragment functions as an activator of transcription in yeast, mammalian cells and in vitro transcription assays. VP16 has been shown to bind to TBP, TFIIB, and replication factor A.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

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This Item
SRP2016G9293C2436
GAL4 [(1-147) + VP16 (411-490)] from Saccharomyces cerevisiae human herpesvirus 2 recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP2017

GAL4 [(1-147) + VP16 (411-490)] from Saccharomyces cerevisiae human herpesvirus 2

GAL4 [(1-147) - AH] from Saccharomyces cerevisiae recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP2016

GAL4 [(1-147) - AH] from Saccharomyces cerevisiae

Anti-GAL4, Activation domain antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

G9293

Anti-GAL4, Activation domain antibody produced in rabbit

Cytochrome c from Saccharomyces cerevisiae ≥85% based on Mol. Wt. 12,588 basis

Sigma-Aldrich

C2436

Cytochrome c from Saccharomyces cerevisiae

description

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

description

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

description

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

description

≥85% based on Mol. Wt. 12,588 basis

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)
human herpesvirus 2 ... HS2VP16A(1487335)

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)

Gene Information

-

Gene Information

Saccharomyces cerevisiae ... CYC1(853507)

assay

≥80% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

-

assay

≥85% based on Mol. Wt. 12,588 basis

technique(s)

electrophoretic mobility shift assay: suitable

technique(s)

electrophoretic mobility shift assay: suitable

technique(s)

western blot: 0.5 μg/mL using GAL4-AD fusion protein in Saccharomyces cerevisiae extract

technique(s)

cell based assay: suitable

biological source

Saccharomyces cerevisiae, human herpesvirus 2

biological source

Saccharomyces cerevisiae

biological source

rabbit

biological source

Saccharomyces cerevisiae

form

frozen liquid

form

frozen liquid

form

buffered aqueous solution

form

powder

concentration

500 μg/mL

concentration

300 μg/mL

concentration

~1.0 mg/mL

concentration

-

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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