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SRP2152

HIV Protease, His tagged,recombinant from HIV-1

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

Synonym(s):

HIV_retropepsin_like, Retropepsins, cd05482, pepsin-like aspartate proteases

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10 μg
Usually ships in 2 weeks.
$577.00

About This Item

NACRES:
NA.26
UNSPSC Code:
12352204
Form:
frozen liquid
Assay:
≥85% (SDS-PAGE)
Biological source:
human immunodeficiency virus 1
Recombinant:
expressed in E. coli
Mol wt:
~11.9 kDa

$577.00

Usually ships in 2 weeks.
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biological source

human immunodeficiency virus 1

recombinant

expressed in E. coli

assay

≥85% (SDS-PAGE)

form

frozen liquid

mol wt

~11.9 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

200 μg/mL

color

colorless to clear

NCBI accession no.

AF459156

UniProt accession no.

Q8JB49

shipped in

dry ice

storage temp.

−70°C

Application

HIV Protease has been used in in vitro detection of HIV protease activity using FRET-HIV Sensor, a Förster resonance energy transfer-based HIV protease-sensitive sensor.

Biochem/physiol Actions

The HIV-1 core consists of a viral genome housed within a conical viral capsid that is generated during virion maturation. Human immunodeficiency virus type 1 (HIV-1) matures after the viral protease processes the Gag and Pol polyproteins at 10 substrate locations. The protease of HIV-1 is an aspartic protease and is functional only as a dimer; dimerization results in the formation of a binding cleft in which each of the two catalytic aspartic acids is contributed by one of the monomers. Because the protease is active only as a dimer, two of the GagPol precursors must themselves dimerize during virus assembly so that their protease domains can dimerize, become active, and process the precursors. The order and kinetics of cleavage as well as the extent of precursor processing appear to be critical steps in the generation of fully infectious, appropriately assembled viral particles. Inhibition of HIV-1 protease represents an important avenue for antiviral therapy. Currently available combination chemotherapy with reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs) for human immunodeficiency virus type 1 (HIV-1) infection and AIDS have been shown to suppress the replication of HIV-1 and extend the life expectancy of HIV-1-infected individuals.

Physical form

Clear and colorless frozen liquid solution
Formulated in 20mM Mes buffer, pH6, 500mM KCl, 20% glycerol.

Preparation Note

Please keep in −20 °C for long term storage. It lost 70% activity in 4°C for one week. Freeze thaw cycle resistance.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Other Notes

PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF

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HIV Protease, His tagged,recombinant from HIV-1 recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

Sigma-Aldrich

SRP2152

HIV Protease, His tagged,recombinant from HIV-1

HIF-1 α N-terminal activation domain (530-698) human recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP2099

HIF-1 α N-terminal activation domain (530-698) human

HIF-1 α, C-terminal activation domain (776-826 human recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

Sigma-Aldrich

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HIF-1 α, C-terminal activation domain (776-826 human

Arginine Splicing Factor, His tagged human recombinant, expressed in insect cells, ≥85% (SDS-PAGE)

Sigma-Aldrich

SRP2104

Arginine Splicing Factor, His tagged human

description

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

description

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

description

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

description

recombinant, expressed in insect cells, ≥85% (SDS-PAGE)

biological source

human immunodeficiency virus 1

biological source

human

biological source

human

biological source

human

assay

≥85% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥85% (SDS-PAGE)

assay

≥85% (SDS-PAGE)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in insect cells

concentration

200 μg/mL

concentration

800 μg/mL

concentration

700 μg/mL

concentration

350 μg/mL

form

frozen liquid

form

frozen liquid

form

frozen liquid

form

frozen liquid

mol wt

~11.9 kDa

mol wt

~19.8 kDa

mol wt

~6.7 kDa

mol wt

~29.3 kDa

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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