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SRP3028

EGF-receptor human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

ErbB1

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10 μg
Usually ships in 2 weeks.
$447.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥95% (HPLC), ≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in CHO cells
Mol wt:
97.5 kDa

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biological source

human

recombinant

expressed in CHO cells

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

mol wt

97.5 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EGFR(1956)

General description

Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor α (TGFα), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin, and epigen. The gene encoding it is localized on human chromosome 7p11.2. Recombinant soluble human EGFR is a 621 amino acid glycoprotein comprising the extracellular domain of EGFR, and migrates at an apparent MW of 97.5 kDa by SDS-PAGE analysis under reducing conditions.

Biochem/physiol Actions

Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

LEEKKVCQGT SNKLTQLGTF EDHFLSLQRM FNNCEVVLGN LEITYVQRNY DLSFLKTIQE VAGYVLIALN TVERIPLENL QIIRGNMYYE NSYALAVLSN YDANKTGLKE LPMRNLQEIL HGAVRFSNNP ALCNVESIQW RDIVSSDFLS NMSMDFQNHL GSCQKCDPSC PNGSCWGAGE ENCQKLTKII CAQQCSGRCR GKSPSDCCHN QCAAGCTGPR ESDCLVCRKF RDEATCKDTC PPLMLYNPTT YQMDVNPEGK YSFGATCVKK CPRNYVVTDH GSCVRACGAD SYEMEEDGVR KCKKCEGPCR KVCNGIGIGE FKDSLSINAT NIKHFKNCTS ISGDLHILPV AFRGDSFTHT PPLDPQELDI LKTVKEITGF LLIQAWPENR TDLHAFENLE IIRGRTKQHG QFSLAVVSLN ITSLGLRSLK EISDGDVIIS GNKNLCYANT INWKKLFGTS GQKTKIISNR GENSCKATGQ VCHALCSPEG CWGPEPRDCV SCRNVSRGRE CVDKCNLLEG EPREFVENSE CIQCHPECLP QAMNITCTGR GPDNCIQCAH YIDGPHCVKT CPAGVMGENN TLVWKYADAG HVCHLCHPNC TYGCTGPGLE GCPTNGPKIP S

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description

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

description

clone 225, purified from hybridoma cell culture

description

recombinant, expressed in HEK 293 cells, clone RM294, purified immunoglobulin

description

Prestige Antibodies® Powered by Atlas Antibodies, clone CL0822, purified immunoglobulin, buffered aqueous glycerol solution

biological source

human

biological source

mouse

biological source

-

biological source

mouse

recombinant

expressed in CHO cells

recombinant

-

recombinant

expressed in HEK 293 cells

recombinant

-

technique(s)

cell culture | mammalian: suitable

technique(s)

immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells

technique(s)

western blot: 1:200-1:500, immunohistochemistry: 1:100-1:200

technique(s)

immunoblotting: 1 μg/mL

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

-

assay

-

assay

-

mol wt

97.5 kDa

mol wt

-

mol wt

-

mol wt

-

form

lyophilized

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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