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Fas Ligand human

Name: Fas Ligand human
Brand: SIGMA
Price: 409 USD
Availability: InStock

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

APTL, Apo I Ligand, CD95L, TNFSF6, soluble Fas Ligand (sFasL)

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Size/SKU	Availability	Price
10 μg	Usually ships in 2 weeks.	$409.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

Form:

lyophilized

Assay:

≥95% (HPLC), ≥95% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in CHO cells

Mol wt:

17.9 kDa

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Properties

biological source

human

recombinant

expressed in CHO cells

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

potency

≤10.0 ng/mL

mol wt

17.9 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

suitability

suitable for molecular biology

UniProt accession no.

P48023

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FASLG(356)

Description

General description

Fas Ligand (FasL) is a member of the TNF (tumor necrosis factor) superfamily that is expressed on the cell surface of activated T cells. It can be present as soluble form in the circulation or membrane bound form in cells. Recombinant human soluble Fas Ligand is a 17.9kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag. Both human and murine sFasL are fully active on human and murine cells.

Application

Fas ligand human has been used to induce apoptosis in T-cell lymphoma-derived HuT78 cells and jurkat cells.

Biochem/physiol Actions

Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-Associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppresses normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

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This Item	F0427	SAB4501538	S8689
Sigma-Aldrich SRP3036 Fas Ligand human	Sigma-Aldrich F0427 Fas Ligand human	Sigma-Aldrich SAB4501538 Anti-FAS LIGAND antibody produced in rabbit	Sigma-Aldrich S8689 Fas Ligand, soluble human
description recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture	description >95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder	description affinity isolated antibody	description recombinant, expressed in HEK 293 cells, lyophilized powder
assay ≥95% (HPLC), ≥95% (SDS-PAGE)	assay >95% (SDS-PAGE)	assay -	assay ≥95% (SDS-GE)
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) ELISA: 1:5000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000	technique(s) ligand binding assay: suitable
recombinant expressed in CHO cells	recombinant expressed in CHO cells	recombinant -	recombinant expressed in HEK 293 cells
mol wt 17.9 kDa	mol wt monomer calculated mol wt ~18 kDa, 26-28 kDa by SDS-PAGE	mol wt antigen 31 kDa	mol wt 32 kDa by SDS-PAGE (nonglycosylated form, under reducing conditions), 35 kDa by SDS-PAGE (glycosylated form, under reducing conditions)
form lyophilized	form lyophilized powder	form buffered aqueous solution	form lyophilized powder
suitability suitable for molecular biology	suitability -	suitability -	suitability -

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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