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IL-10 human

Name: IL-10 human
Brand: SIGMA
Price: 367 USD
Availability: InStock

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture

Synonym(s):

B-TCGF, CSIF, TGIF

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Size/SKU	Availability	Price
10 μg	Usually ships in 2 weeks.	$367.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

$367.00

Usually ships in 2 weeks.

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Properties

biological source

human

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL ED₅₀

mol wt

18.8 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P22301

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL10(3586)

Description

General description

IL-10 (interleukin 10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. The gene is mapped to human chromosome 1q31-1q32. Recombinant human IL-10 is an 18.6kDa protein of 161 amino acid residues. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells.

Application

IL-10 human has been used to study the effect of IL-10 on endothelial nitric oxide synthase and nitric oxide in African American human umbilical vein endothelial cells. It has also been used to study the effect of IL-10 on leukotriene (LT) A4 hydrolase and LTC4 synthase protein expression in and secretion from, an inflamed porcine endometrium.

Biochem/physiol Actions

IL-10 (interleukin 10) is an anti-inflammatory cytokine. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF (tumor necrosis factor)-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. Polymorphism in IL-10 at -1082A/G is associated with diabetic nephropathy.

Physical form

Lyophilized from a 0.2 μm filtered solution containing10 mM Sodium Phosphate, pH 7.5

Preparation Note

Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Other Notes

MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

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Show Differences

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This Item	SRP3312	SAB5700775	SAB5702039
Sigma-Aldrich SRP3071 IL-10 human	Sigma-Aldrich SRP3312 IL-10 human	Sigma-Aldrich SAB5700775 Anti-IL10 antibody produced in rabbit	Sigma-Aldrich SAB5702039 Anti-IL10 Antibody, clone 6U0G7, Rabbit Monoclonal
description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture	description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)	description -	description -
assay ≥98% (SDS-PAGE)	assay ≥98% (SDS-PAGE), ≥98% (HPLC)	assay -	assay -
biological source human	biological source human	biological source rabbit	biological source rabbit
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) western blot: 1:500-1:2000, immunohistochemistry: 1:50-1:100	technique(s) western blot: 1:500 - 1:2000, immunofluorescence: 1:50 - 1:200
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant -	recombinant -
form lyophilized	form lyophilized	form buffered aqueous solution	form liquid
mol wt 18.8 kDa	mol wt 18.6 kDa	mol wt 20	mol wt 18 kDa

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

110621

0903B21-3

0903B21-2

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