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SRP3117

MMP-1 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Fibroblast collagenase

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10 μg
Usually ships in 2 weeks.
$402.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Assay:
≥98% (HPLC), ≥98% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli

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biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

mol wt

42.7 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP1(4312)

General description

Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. MMP-1 is expressed in various cells, including fibroblasts, keratinocytes, chondrocytes, monocytes and macrophages, hepatocytes and many tumor cells. The gene is mapped to human chromosome 11q22. Recombinant human MMP-1 is a 42.7kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain which is involved in substrate specificity and in binding TIMP-1 (tissue inhibitor of metalloproteinases 1).

Application

MMP-1 (matrix metalloproteinase 1) human has been used to study effect of NF (nuclear factor)κB-mediated expression of MMP-1 in breast cancer spheroids.

Biochem/physiol Actions

Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens, aggrecan, serpins, and α2-macroglobulin. It is overexpressed in lumbar intervertebral disc degeneration (IDD). It might also be associated with osteoarthritis. MMP-1 is linked with Jjuvenile idiopathic arthritis. Genetic variations in MMP-1 can affect the susceptibility and severity of this disease.

Physical form

Lyophilized from 20 mM TRIS, pH 9.0 + 0.1 mM Calcium Chloride + 100 mM Arginine.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG PGIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGALMYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DSKLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPNGL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFPRTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAHD FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANSWFNCRK N

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MMP-1 human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3117

MMP-1 human

Anti-MMP-1 Antibody, a.a. 332-350 hMMP1, clone 41-1E5 clone 41-1E5, Chemicon&#174;, from mouse

Sigma-Aldrich

MAB3307

Anti-MMP-1 Antibody, a.a. 332-350 hMMP1, clone 41-1E5

Anti-Matrix Metalloproteinase-1 antibody produced in goat affinity isolated antibody, lyophilized powder

Sigma-Aldrich

M4696

Anti-Matrix Metalloproteinase-1 antibody produced in goat

Anti-MMP-1 Antibody, hinge region from rabbit, purified by affinity chromatography

Sigma-Aldrich

AB6002

Anti-MMP-1 Antibody, hinge region

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

clone 41-1E5, Chemicon®, from mouse

description

affinity isolated antibody, lyophilized powder

description

from rabbit, purified by affinity chromatography

Gene Information

human ... MMP1(4312)

Gene Information

human ... MMP1(4312)

Gene Information

human ... MMP1(4312)

Gene Information

human ... MMP1(4312)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

-

assay

-

assay

-

biological source

human

biological source

mouse

biological source

goat

biological source

rabbit

technique(s)

cell culture | mammalian: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable, ELISA: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-15 μg/mL, immunocytochemistry: suitable, western blot: 0.1-0.2 μg/mL

technique(s)

western blot: suitable

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

lyophilized

form

-

form

lyophilized powder

form

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    1110439
    0610439
    0508439
    0108439
    0815439-1

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