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TIMP-1 human

Name: TIMP-1 human
Brand: SIGMA
Price: 395 USD
Availability: InStock

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

Erythroid-Potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinase

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Size/SKU	Availability	Price
10 μg	Usually ships in 2 weeks.	$395.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

Form:

lyophilized

Assay:

≥95% (HPLC), ≥95% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in E. coli

Mol wt:

20.6 kDa

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Properties

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

potency

0.5 μg/mL

mol wt

20.6 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

suitability

suitable for molecular biology

UniProt accession no.

P01033

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TIMP1(7076)

Description

General description

Research area: Cell SignalingTIMP1 (tissue inhibitor of metalloproteinase 1) is a glycoprotein and belongs to the TIMP family of endogenous MMP (matrix metalloproteinase) inhibitors. Humans contain four TIMPs. All TIMPs contain an N-terminal of 125 residues, a 65-residue C-terminal, and both these domains with three disulfide bonds. The N-terminal domain inhibits MMPs by folding into a separate unit. Recombinant human TIMP-1 is a 20.6 kDa protein containing 184 amino acid residues.

Application

TIMP-1 human has been used for inhibition assays in enzymatic activity assays of Mmp1-CD and Mmp2-CD. It has also been used in interaction immunoassay.

Biochem/physiol Actions

TIMP1 functions as a poor inhibitor of MT1 (membrane type 1)-MMP, MT3-MMP, MT5-MMP and MMP-19. It inhibits ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10) protein. In HUVECs, the down-regulation of TIMP1 and up-regulation of MMP-3 results in aberrant endothelium-dependent vasodilation, EC (endothelial cell) death, and endothelial interruption in a FOXO3 (forkhead box O3)-mediated manner. In patients with CAD (coronary artery disease) and acute coronary syndrome (ACS), the urine levels of this protein are elevated. This protein interacts with Bcl-2 (B cell lymphoma) protein, and induces apoptosis in lung adenocarcinoma cells. The plasma levels of this protein are increased in patients with obesity and obesity-related disorders, such as steatosis, where it participates in pathogenesis of diet-induced hepatic steatosis and glucose intolerance.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA

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Show Differences

1 of 1

This Item	MABC1142
Sigma-Aldrich SRP3173 TIMP-1 human	Sigma-Aldrich MABC1142 Anti-TIMP-4 Antibody, clone 9:4-7
description recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture	description clone 4-7, from mouse
biological source human	biological source mouse
assay ≥95% (SDS-PAGE), ≥95% (HPLC)	assay -
technique(s) cell culture \| mammalian: suitable	technique(s) immunohistochemistry: suitable (paraffin), ELISA: suitable, western blot: suitable
recombinant expressed in E. coli	recombinant -
form lyophilized	form -
mol wt 20.6 kDa	mol wt -

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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