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SRP3173

TIMP-1 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

Erythroid-Potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinase

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10 μg
Usually ships in 2 weeks.
$409.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥95% (HPLC), ≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
20.6 kDa

$409.00

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biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

potency

0.5 μg/mL

mol wt

20.6 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

suitability

suitable for molecular biology

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TIMP1(7076)

General description

Research area: Cell SignalingTIMP1 (tissue inhibitor of metalloproteinase 1) is a glycoprotein and belongs to the TIMP family of endogenous MMP (matrix metalloproteinase) inhibitors. Humans contain four TIMPs. All TIMPs contain an N-terminal of 125 residues, a 65-residue C-terminal, and both these domains with three disulfide bonds. The N-terminal domain inhibits MMPs by folding into a separate unit. Recombinant human TIMP-1 is a 20.6 kDa protein containing 184 amino acid residues.

Application

TIMP-1 human has been used for inhibition assays in enzymatic activity assays of Mmp1-CD and Mmp2-CD. It has also been used in interaction immunoassay.

Biochem/physiol Actions

TIMP1 functions as a poor inhibitor of MT1 (membrane type 1)-MMP, MT3-MMP, MT5-MMP and MMP-19. It inhibits ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10) protein. In HUVECs, the down-regulation of TIMP1 and up-regulation of MMP-3 results in aberrant endothelium-dependent vasodilation, EC (endothelial cell) death, and endothelial interruption in a FOXO3 (forkhead box O3)-mediated manner. In patients with CAD (coronary artery disease) and acute coronary syndrome (ACS), the urine levels of this protein are elevated. This protein interacts with Bcl-2 (B cell lymphoma) protein, and induces apoptosis in lung adenocarcinoma cells. The plasma levels of this protein are increased in patients with obesity and obesity-related disorders, such as steatosis, where it participates in pathogenesis of diet-induced hepatic steatosis and glucose intolerance.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA

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TIMP-1 human recombinant, expressed in E. coli, &#8805;95% (SDS-PAGE), &#8805;95% (HPLC), suitable for cell culture

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TIMP-1 human

Anti-TIMP-1 Antibody Chemicon&#174;, from rabbit

Sigma-Aldrich

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Anti-TIMP-1 Antibody, clone 102D1 clone 102D1, Chemicon&#174;, from mouse

Sigma-Aldrich

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Anti-TIMP-1 Antibody, clone 102D1

Human TIMP-1 ELISA Kit for serum, plasma, cell culture supernatant and urine

Sigma-Aldrich

RAB0466

Human TIMP-1 ELISA Kit

description

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

description

Chemicon®, from rabbit

description

clone 102D1, Chemicon®, from mouse

description

for serum, plasma, cell culture supernatant and urine

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

biological source

human

biological source

rabbit

biological source

mouse

biological source

-

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

-

assay

-

assay

-

technique(s)

cell culture | mammalian: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable, ELISA: suitable

technique(s)

western blot: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

capture ELISA: suitable

suitability

suitable for molecular biology

suitability

-

suitability

-

suitability

-

form

lyophilized

form

-

form

-

form

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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