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SRP3174

TIMP-2 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

CSC-21K, Tissue inhibitor of metalloproteinase 2

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10 μg
Usually ships in 2 weeks.
$402.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$402.00

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biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

potency

0.5 μg/mL

mol wt

21.8 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TIMP2(7077)

General description

Tissue inhibitor of metalloproteinase-2 (TIMP-2) is an extracellular inhibitor of matrix metalloproteinases (MMPs) including MMP1, 2, 3, 7, 8, 9, 10, 12, 13, 14, 15, 16 and 19. It belongs to the I35 (TIMP) family of irreversible protease inhibitors. The gene encoding this protein is localized on human chromosome 17q25. Recombinant human TIMP-2 is a 21.8kDa protein containing 194 amino acid residues.

Biochem/physiol Actions

Tissue inhibitor of metalloproteinase-2 (TIMP-2), like other protease inhibitors, functions as a key modulator of extracellular matrix degradation during tissue development and remodeling. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. It demonstrates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

MCSCSPVHPQ QAFCNADVVI RAKAVSEKEV DSGNDIYGNP IKRIQYEIKQ IKMFKGPEKD IEFIYTAPSS AVCGVSLDVG GKKEYLIAGK AEGDGKMHIT LCDFIVPWDT LSTTQKKSLN HRYQMGCECK ITRCPMIPCY ISSPDECLWM DWVTEKNING HQAKFFACIK RSDGSCAWYR GAAPPKQEFL DIEDP

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TIMP-2 human recombinant, expressed in E. coli, &#8805;95% (SDS-PAGE), &#8805;95% (HPLC), suitable for cell culture

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Human TIMP-2 ELISA Kit for serum, plasma, cell culture supernatant and urine

Sigma-Aldrich

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Anti-TIMP2 antibody produced in rabbit

Sigma-Aldrich

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Anti-TIMP2 antibody produced in rabbit

Anti-TIMP2 antibody, Mouse monoclonal clone TM8, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200814

Anti-TIMP2 antibody, Mouse monoclonal

description

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

description

for serum, plasma, cell culture supernatant and urine

description

-

description

clone TM8, purified from hybridoma cell culture

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

-

assay

-

assay

-

biological source

human

biological source

-

biological source

rabbit

biological source

mouse

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

immunofluorescence: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

immunofluorescence: 2.5-5 μg/mL using human HeLa cells., immunoblotting: 0.5-1 μg/mL using HEK-293 over expressing human TIMP2 cells extract.

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

lyophilized

form

-

form

buffered aqueous solution

form

-

UniProt accession no.

P16035

UniProt accession no.

-

UniProt accession no.

P16035

UniProt accession no.

P16035

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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