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EGF from mouse

Name: EGF from mouse
Brand: SIGMA
Price: 380 USD
Availability: InStock

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):

Epidermal Growth Factor, URG, Urogastrone

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Size/SKU	Availability	Price
500 μg	Usually ships in 2 weeks.	$380.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

$380.00

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Properties

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

0.1 ng/mL

mol wt

6.0 kDa

packaging

pkg of 500 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

UniProt accession no.

P01132

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... EGF(13645)

Description

General description

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.

Application

EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.

Biochem/physiol Actions

EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 8.2.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

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This Item	SRP3238	SRP3027	GF316
Sigma-Aldrich SRP3196 EGF from mouse	Sigma-Aldrich SRP3238 EGF from rat	Sigma-Aldrich SRP3027 EGF human	Sigma-Aldrich GF316 EGF Protein, Human Recombinant Animal Free
description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture	description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture	description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture	description This Recombinant animal free human EGF is manufactured using all non-animal reagents.
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
assay ≥98% (SDS-PAGE), ≥98% (HPLC)	assay ≥98% (SDS-PAGE), ≥98% (HPLC)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay >98% (SDS-PAGE and HPLC)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
biological source mouse	biological source rat	biological source human	biological source -
form lyophilized	form lyophilized	form lyophilized	form -
Gene Information mouse ... EGF(13645)	Gene Information rat ... EGF(25313)	Gene Information human ... EGF(1950)	Gene Information -

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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