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SRP3196

EGF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Epidermal Growth Factor, URG, Urogastrone

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500 μg
Usually ships in 2 weeks.
$388.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$388.00


Usually ships in 2 weeks.

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biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

0.1 ng/mL

mol wt

6.0 kDa

packaging

pkg of 500 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... EGF(13645)

General description

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.

Application

EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.

Biochem/physiol Actions

EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 8.2.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

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SRP3329SRP3238SRP3027
description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

biological source

mouse

biological source

mouse

biological source

rat

biological source

human

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

UniProt accession no.

P01132

UniProt accession no.

P01133

UniProt accession no.

P07522

UniProt accession no.

P01133


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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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