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NOGGIN from mouse

Name: NOGGIN from mouse
Brand: SIGMA
Price: 389 USD
Availability: InStock

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Mouse noggin, NOGGIN growth factor, NOGGIN protein

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20 μg	Usually ships in 2 weeks.	$389.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

$389.00

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Properties

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-2.0 ng/mL ED₅₀

mol wt

46.4 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P97466

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... NOG(18121)

Description

General description

NOGGIN was first identified in the Xenopus embryos in an expression screen for activities that induce dorsal structures. It is a glycoprotein that is released as a homodimer. This protein is expressed during Xenopus gastrula stage. NOGGIN shows major expression in the central nervous system and is also expressed in lung, skin, skeletal muscle, cartilage, and bone. Recombinant murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.

Application

NOGGIN from mouse has been used as a supplement in the knockout serum replacement constituting the embryoid body medium containing DMEM/F-12. It has also been used as a supplement in PPC (photoreceptor progenitor cell) intermediate medium.

Biochem/physiol Actions

NOGGIN proteins interact with BMPs (bone morphogenetic protein) and inhibit the activation of BMPRs. In Xenopus gastrula stage, NOGGIN is released by the Spemann organizer, and stimulates neural tissue from dorsal ectoderm by inhibiting ectodermal BMPs. In mouse embryo, this protein is not essential for neural induction, but is crucial for the later development of the neural tube, somite, and cartilage morphogenesis. Double homozygous mutant mice of NOGGIN and CHORDIN show prosencephalon developmental defects. In vitro it functions as a negative regulator of neuronal differentiation of neocortical precursors.

Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. Recombinant murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

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This Item	N6784	N17001	GF173
Sigma-Aldrich SRP3227 NOGGIN from mouse	Sigma-Aldrich N6784 Noggin/Fc Chimera from mouse	Sigma-Aldrich N17001 Noggin human	Sigma-Aldrich GF173 Noggin Protein, Human Recombinant
description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture	description >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder	description recombinant, expressed in HEK 293 cells, suitable for cell culture	description The human Noggin recombinant protein belongs to a group of diffusible proteins which bind to ligands of the TGF-β family & regulate their activity by inhibiting their access to signaling receptors.
recombinant expressed in E. coli	recombinant expressed in NSO cells	recombinant expressed in HEK 293 cells	recombinant expressed in HEK 293 cells
biological source mouse	biological source mouse	biological source -	biological source -
assay ≥98% (SDS-PAGE), ≥98% (HPLC)	assay >95% (SDS-PAGE)	assay ≥98% (SDS-PAGE)	assay >95% (SDS-PAGE and HPLC)
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable	technique(s) -
form lyophilized	form lyophilized powder	form lyophilized powder	form -
UniProt accession no. P97466	UniProt accession no. P97466	UniProt accession no. -	UniProt accession no. Q13253

Storage Class

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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