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SRP3299

GDF-5 mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

BMP-14, CDMP-1

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50 μg
Usually ships in 2 weeks.
$392.00
$401.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$401.00

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biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

0.08-0.12 μg/mL

mol wt

27.0 kDa

packaging

pkg of 50 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... GDF5(14563)

General description

Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 348 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.

Biochem/physiol Actions

GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Recombinant murine GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

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SRP3200SRP3309G3046
description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

-

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in CHO cells

biological source

mouse

biological source

mouse

biological source

human

biological source

human

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>97% (visualized with silver stain, SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized powder

impurities

<0.1 EU/μg endotoxin, tested

impurities

<0.1 EU/μg endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

mol wt

27.0 kDa

mol wt

15.1 kDa

mol wt

30.0 kDa

mol wt

dimer 13 kDa (based on N-terminal sequencing, starting at the first histidine of the 6X histidine tag)


Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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