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SRP5034

GRK2, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

β-ARK1, ADRBK1, BARK1, FLJ16718

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10 μg
Usually ships in 2 weeks.
$596.00

About This Item

NACRES:
NA.77
UNSPSC Code:
12352200
Specific activity:
4.5-6.1 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells

$596.00

Usually ships in 2 weeks.
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recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

4.5-6.1 nmol/min·mg

mol wt

~102 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ADRBK1(156)

General description

G-protein-coupled receptor kinase 2 (GRK2), which is officially known as adrenergic, beta, receptor kinase 1 (ADRBK1), gene codes for a 689 amino acid protein, that is of 79.573kDa. This gene has 12 catalytic region subdomains. GRK2 is expressed in the vascular endothelium, arterial smooth muscle, myocardium, kidney and in the renal proximal tubule. It is mapped to human chromosome 11q13.

Biochem/physiol Actions

G-protein-coupled receptor kinase 2 (GRK2) or ADRBK1 is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G protein-coupled receptors. GRK2 phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. RAF kinase inhibitor protein RKIP is a physiologic inhibitor of GRK2. After stimulation of G protein-coupled receptors, RKIP dissociates from its known target, RAF1 to associate with GRK2 and block its activity. G-protein-coupled receptor kinase 2 (GRK2) controls several intracellular mechanisms. It helps to maintain the structure and activities of the heart. Hence it participates in several cardiovascular diseases. GRK2 also modulates ion and fluid transport and eventually, blood pressure. It increases the synthesis of mitochondrial superoxide and reduces oxygen utilization and ATP synthesis. This protein serves as a major onco-modulator.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

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GRK2, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

SRP5034

GRK2, active, GST tagged human

Anti-ADRBK1 antibody produced in rabbit

Sigma-Aldrich

SAB5700131

Anti-ADRBK1 antibody produced in rabbit

Anti-GRK2 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody

Sigma-Aldrich

HPA048330

Anti-GRK2 antibody produced in rabbit

GRK1, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

SRP5033

GRK1, active, GST tagged human

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

-

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

assay

≥70% (SDS-PAGE)

assay

-

assay

-

assay

≥70% (SDS-PAGE)

Gene Information

human ... ADRBK1(156)

Gene Information

human ... ADRBK1(156)

Gene Information

human ... ADRBK1(156)

Gene Information

human ... GRK1(6011)

specific activity

4.5-6.1 nmol/min·mg

specific activity

-

specific activity

-

specific activity

3.6-4.9 nmol/min·mg

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

-

recombinant

-

recombinant

expressed in baculovirus infected Sf9 cells

shipped in

dry ice

shipped in

-

shipped in

wet ice

shipped in

-

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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