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SRP5047

MEKK1 (664-1512), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

MAP3K1, MAPKKK1, MEKK

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10 μg
Usually ships in 2 weeks.
$593.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
697-943 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells

$593.00


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recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

697-943 nmol/min·mg

mol wt

~155 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... Map3K1(4214)

General description

MEKK1 or MEK kinase, is a serine/threonine kinase that is downstream of mitogenic and metabolic stimuli, including insulin and many growth factors. MEKK1 functions not only as an upstream activator of ERK and JNK through its kinase domain, but also as an E3 ligase through its PHD domain, providing a negative regulatory mechanism for decreasing ERK1/ERK2 activity by ubiquitination and degradation. MEKK1 -/- embryonic stem cells from mice show loss or altered responses of JNK to microtubule disruption and cold stress. Furthermore, activation of JNK is lost and that of ERK is diminished in response to hyperosmolarity and serum factors in MEKK1 -/- cells.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

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HPA046509M0449WH0004214M1
description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

clone 2F6, purified immunoglobulin, buffered aqueous solution

assay

≥70% (SDS-PAGE)

assay

-

assay

≥70% (SDS-PAGE)

assay

-

Gene Information

human ... Map3K1(4214)

Gene Information

human ... MAP3K1(4214)

Gene Information

human ... MAP3K3(4215)

Gene Information

human ... MAP3K1(4214)

specific activity

697-943 nmol/min·mg

specific activity

-

specific activity

17-23 nmol/min·mg

specific activity

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

-

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

-

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice


Storage Class

10 - Combustible liquids



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