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TRAIL, Soluble human

Name: TRAIL, Soluble human
Brand: SIGMA
Price: 631 USD

recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

Synonym(s):

Apo-2L, CD253, CD339, TRAIL

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Pack Size	SKU	Availability	Price
20 μg		Usually ships in 2 weeks.	$631.00

About This Item

NACRES:

NA.77

UNSPSC Code:

12352200

Form:

liquid

Assay:

>98% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in E. coli

Mol wt:

~26 kDa

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Properties

biological source

human

recombinant

expressed in E. coli

tag

His tagged

assay

>98% (SDS-PAGE)

form

liquid

mol wt

~26 kDa

packaging

pkg of 20 μg

concentration

≥0.2 mg/mL

technique(s)

activity assay: suitable, cell culture | mammalian: suitable, transfection: suitable

impurities

<0.01 EU/μg endotoxin, tested

color

clear

UniProt accession no.

P50591

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TNFSF10(8743)

Description

General description

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

Application

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

Biochem/physiol Actions

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

Physical form

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

Other Notes

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

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Show Differences

1 of 1

This Item	GF092	T9701	SRP8025
Sigma-Aldrich SRP8024 TRAIL, Soluble human	Sigma-Aldrich GF092 TRAIL Protein, Recombinant human	Sigma-Aldrich T9701 TRAIL human	Sigma-Aldrich SRP8025 TRAIL, Soluble mouse
assay >98% (SDS-PAGE)	assay >98% (SDS-PAGE and HPLC)	assay ≥98% (SDS-PAGE and HPLC)	assay >95% (SDS-PAGE)
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
technique(s) activity assay: suitable, transfection: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) immunofluorescence: suitable	technique(s) -
biological source human	biological source human	biological source human	biological source mouse
concentration ≥0.2 mg/mL	concentration -	concentration -	concentration ≥0.2 mg/mL
form liquid	form solid	form lyophilized powder	form liquid

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Genome-Scale MicroRNA and Small Interfering RNA Screens Identify Small RNA Modulators of TRAIL-Induced Apoptosis Pathway

Dmitriy Ovcharenko

Cancer Research (2007)

Association of TRAIL and Its Receptors with Large-Artery Atherosclerotic Stroke.

Pan X

PLoS ONE, 10(9), e0136414-e0136414 (2015)

Intracranial AAV-sTRAIL combined with lanatoside C prolongs survival in an orthotopic xenograft mouse model of invasive glioblastoma.

Crommentuijn MH

Molecular Oncology, 10(4), 625-634 (2016)

View All Related Papers

Global Trade Item Number

SKU	GTIN
SRP8024-20UG	04061837313684

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