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SRP8043

APRIL, Soluble mouse

recombinant, expressed in HEK 293 cells, FLAG® tagged, >95% (SDS-PAGE)

Synonym(s):

ACRP30headless:A-proliferation-inducing ligand, ACRP30headless:APRIL, ACRP30headless:CD256, TNFSF10

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Size/SKUAvailabilityPrice
10 μg
Usually ships in 2 weeks.
$511.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
>95% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in HEK 293 cells
Mol wt:
~34 kDa by SDS-PAGE

$511.00


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biological source

mouse

recombinant

expressed in HEK 293 cells

tag

FLAG® tagged

assay

>95% (SDS-PAGE)

form

lyophilized

mol wt

~34 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.02 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Tnfsf13(69583)

General description

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. APRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

Physical form

Lyophilized from PBS.

Preparation Note

Reconstitute with 100 μL deionized water.

Other Notes

The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-110) and a FLAG®-tag.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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SRP3189SRP8048SRP8041
description

recombinant, expressed in HEK 293 cells, FLAG® tagged, >95% (SDS-PAGE)

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in CHO cells, FLAG® tagged, >95% (SDS-PAGE)

description

recombinant, expressed in CHO cells, >95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in CHO cells

recombinant

expressed in CHO cells

form

lyophilized

form

lyophilized

form

lyophilized

form

liquid

mol wt

~34 kDa by SDS-PAGE

mol wt

21.9 kDa

mol wt

~47 kDa by SDS-PAGE

mol wt

monomer ~180 kDa by SDS-PAGE (non-reducing), monomer ~80 kDa by SDS-PAGE (reducing)

impurities

<0.02 EU/μg endotoxin, tested

impurities

<0.1 EU/μg endotoxin, tested

impurities

<0.01 EU/μg endotoxin, tested

impurities

<1 EU/μg endotoxin, tested


Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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