The sequence of this product is not mapped.
T0706
Tropoelastin human
recombinant, expressed in E. coli, 1 mg
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| Size/SKU | Availability | Price |
|---|---|---|
1 mg | Check Cart for Availability | $584.00 |
About This Item
UNSPSC Code:
12352202
EC Number:
232-701-4
NACRES:
NA.75
Recombinant:
expressed in E. coli
Impurities:
≤10 EU/mL Endotoxin
$584.00
Check Cart for Availability
recombinant
expressed in E. coli
Quality Segment
description
1 mg recombinant human Tropoelastin as lyophilized powder.
sterility
electron beam irradiated
concentration
1 mg
impurities
≤10 EU/mL Endotoxin
storage temp.
−20°C
General description
Tropoelastin is a 60-72 kDa alternatively spliced extracellular matrix protein. It is a soluble precursor of elastin and is distributed in elastic tissues. Tropoelastin consists of an N- terminal 26-amino-acid signal peptide and highly basic strongly conserved C-terminal domain, that is required for both incorporation into elastin and for cellular interactions. It Is also characterized with a hydrophobic domain and exon 26A, which is an unusual highly hydrophilic domain.
Application
Preparation Procedure
Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Add 1 ml of sterile 0.25% glacial acetic acid solution to the tropoelastin serum vial containing 1 mg.
2. Mix contents gently until material is completely solubilized. The solution may remain slightly hazy.
3. Transfer desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to desired concentration using sterile 0.25% Acetic Acid solution. A typical working concentration may range from 1 to 50 μg/ml.
4. Add appropriate amount of diluted tropoelastin material to the culture surface.
5. Incubate at room temperature or 37 °C, covered, for approximately 1.5 hours.
6. After incubation, aspirate any remaining material.
7. Rinse coated surfaces carefully with sterile water and avoid scratching surfaces.
8. Coated surfaces are ready for use. They may also be stored at 2-10 °C damp or air dried if sterility is maintained.
Note: To maintain sterility, perform all operations in a laminar flow hood employing aseptic techniques.
Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Add 1 ml of sterile 0.25% glacial acetic acid solution to the tropoelastin serum vial containing 1 mg.
2. Mix contents gently until material is completely solubilized. The solution may remain slightly hazy.
3. Transfer desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to desired concentration using sterile 0.25% Acetic Acid solution. A typical working concentration may range from 1 to 50 μg/ml.
4. Add appropriate amount of diluted tropoelastin material to the culture surface.
5. Incubate at room temperature or 37 °C, covered, for approximately 1.5 hours.
6. After incubation, aspirate any remaining material.
7. Rinse coated surfaces carefully with sterile water and avoid scratching surfaces.
8. Coated surfaces are ready for use. They may also be stored at 2-10 °C damp or air dried if sterility is maintained.
Note: To maintain sterility, perform all operations in a laminar flow hood employing aseptic techniques.
Biochem/physiol Actions
Tropoelastin exhibits the property of coacervation and plays a vital role in fiber formation and assembly. Abnormality in the tropoelastin is associated with the development of hereditary disease, cutis laxa (where the elasticity of the skin is lost).
Features and Benefits
Tropoelastin available in its native state. Unique since it becomes cross-linked immediately after its synthesis by the cell and during its export into the extracellular matrix.
Analysis Note
Identity and purity qualitatively determined by SDS-PAGE
Other Notes
Storage at -20 °C prior to solubilization is recommended. Storage at 2-10 °C after reconstitution is recommended.
Shelf life is 24 months when stored at -20 °C. Shelf life after reconstitution is 3 months when stored at 2-10 °C
Shelf life is 24 months when stored at -20 °C. Shelf life after reconstitution is 3 months when stored at 2-10 °C
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, 1 mg | description recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture | description recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE) | description recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC) |
| recombinant expressed in E. coli | recombinant expressed in HEK 293 cells | recombinant expressed in baculovirus infected insect cells | recombinant expressed in CHO cells |
| concentration 1 mg | concentration - | concentration >0.02 mg/mL | concentration - |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| impurities ≤10 EU/mL Endotoxin | impurities <0.1 EU/μg endotoxin, tested | impurities - | impurities endotoxin, tested |
| description 1 mg recombinant human Tropoelastin as lyophilized powder. | description - | description - | description - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hi, Can you send me the sequence of this protein? do you have any peptide mapping or data confirming the sequence?
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