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Anti-Tumor Necrosis Factor-α antibody produced in goat

Name: Anti-Tumor Necrosis Factor-α antibody produced in goat
Brand: SIGMA
Price: 651 USD
Availability: InStock

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-TNF-α

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Size/SKU	Availability	Price
0.1 mg	Check Cart for Availability	$651.00

About This Item

NACRES:

NA.41

UNSPSC Code:

51111800

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, Neutral, WB

Citations:

$651.00

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Properties

biological source

goat

Quality Segment

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

immunohistochemistry: suitable, neutralization: suitable, western blot: suitable

UniProt accession no.

P01375

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

Description

General description

Tumor Necrosis Factor-α (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of TNFR super family. The TNF/TNFR signaling axis plays a critical role in mediating either survival or apoptosis depending on the class of adaptor proteins recruited in the cell. Association of receptors with a death domain causes rapid activation of caspases and results in cell death. TNF/TNFR also results in the activation of downstream pathways involving MAPK, p38, JNK and NF-κB that play a major role in innate immunity, acute inflammatory responses and homeostasis. The TNF/TNFR axis is also important in the pathology of various autoimmune and inflammatory disorders including a variety of malignancies and Alzheimer′s disease. Anti-Tumor Necrosis Factor-α antibody is specific for human TNF-α. The antibody shows less than 10% cross reactivity with recombinant rabbit TNF-α and less than 5% cross reactivity with recombinant mouse TNF-α.

Immunogen

recombinant human TNF-α.

Application

Anti-Tumor Necrosis Factor-α (TNF- α) antibody produced in goat has been used to study the release of TNF- α from the cocultured lung carcinoma cells A427:AML-193.

Anti-Tumor Necrosis Factor-α antibody may be used for immunoblotting at a working antibody concentration of 0.1 μg/ml. For immunohistochemistry a concentration range of 5-15 μg/ml is recommended. The antibody may be used for neutralization assay; the ND50 is 0.01-0.06 μg/mL in the presence of 1.5ng/mL Recombinant Human TNF-alpha.

Biochem/physiol Actions

The antibody neutralizes the biological activity of recombinant human TNF-α. The antibody shows <10% cross-reactivity with recombinant rat TNF-α and <5% cross-reactivity with recombinant mouse TNF-α.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Preparation Note

The antibody is purified using TNF-α affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	AB1837P	T6817	T1816
Sigma-Aldrich T0813 Anti-Tumor Necrosis Factor-α antibody produced in goat	Sigma-Aldrich AB1837P Anti-Tumor Necrosis Factor-α Antibody	Sigma-Aldrich T6817 Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in mouse	Sigma-Aldrich T1816 Anti-Tumor Necrosis Factor-α antibody produced in goat
description affinity isolated antibody, lyophilized powder	description Chemicon^®, from rabbit	description clone 28401, purified immunoglobulin, lyophilized powder	description affinity isolated antibody, lyophilized powder
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
biological source goat	biological source rabbit	biological source mouse	biological source goat
technique(s) western blot: suitable, neutralization: suitable, immunohistochemistry: suitable	technique(s) ELISA: suitable, western blot: suitable	technique(s) western blot: 1.0-2.0 μg/mL, neutralization: suitable, capture ELISA: 4 μg/mL, immunocytochemistry: 8-25 μg/mL using Immersion fixed human peripheral blood mononuclear cells (PBMSs) stimulated with LPS and monensin.	technique(s) neutralization: suitable, immunohistochemistry: 15 μg/mL, western blot: 0.1-0.2 μg/mL, indirect ELISA: 0.5-1.0 μg/mL
clone polyclonal	clone polyclonal	clone 28401, monoclonal	clone polyclonal

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Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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