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T1192
Anti-Thymine Dimer antibody, Mouse monoclonal
clone H3, purified from hybridoma cell culture
Synonym(s):
Mouse Anti-Thymine Dimer, Thymine Dimer Detection, Thymine Dimer Mouse Antibody
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| Size/SKU | Availability | Price |
|---|---|---|
25 μL | Available to ship TODAYfromMILWAUKEE | $194.00 |
0.1 mL | Available to ship TODAYfromMILWAUKEE | $926.00 |
About This Item
Conjugate:
unconjugated
Clone:
H3, monoclonal
Application:
DB, ELISA (c), ICC
Citations:
7
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
H3, monoclonal
form
buffered aqueous solution
species reactivity
chicken, wide range
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
capture ELISA: suitable, dot blot: 0.5-1 μg/mL, immunocytochemistry: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
UV light induces the formation of thymine dimers that subsequently leads to DNA damage in cells. Monoclonal antibodies that bind to thymine dimers enable the study of cell death and UV-induced DNA damage during photocarcinogenesis . Monoclonal Anti-Thymine Dimer binds to the (5′-6′) cyclobutane type of homothymine or thyminecytosine heterodimers in single-stranded DNA. The antibody has a decreased affinity for the dimer in short oligonucleotides (an oligonucleotide of atleast 10-20 thymine residues is required for the labeling of the probes). The product facilitates the use of a sensitive and non-radioactive technique for the analysis of thymine dimerization.
Immunogen
tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.
Application
May be used in Southern blotting at 0.5-1μg/mL using UV induced thymine dimers of λ phage DNA fragments.
Monoclonal Anti-Thymine Dimer antibody is suitable for use in dot blot (0.5-1 mg/mL), immunocytochemistry (1:100) and capture ELISA.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Other Notes
Distributed under license.
Patents WO87/01134, EP 0233 177 B1
Patents WO87/01134, EP 0233 177 B1
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone H3, purified from hybridoma cell culture | description clone HAP46.5, purified from hybridoma cell culture | description clone BU-33, ascites fluid, Immunohistology Grade | description clone 11G5, purified from hybridoma cell culture |
| clone H3, monoclonal | clone HAP46.5, monoclonal | clone BU-33, monoclonal | clone 11G5, monoclonal |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form ascites fluid | antibody form purified immunoglobulin |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| species reactivity chicken, wide range | species reactivity canine, mouse, rat, chicken, human | species reactivity wide range | species reactivity Xenopus, human |
| isotype IgG1 | isotype IgG1 | isotype IgG1 | isotype IgG1 |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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When using PN T1192 as a primary antibody (1/200) on UV irradiated skin samples (IHC, Fresh frozen), I get no signal despite using a fluorescently labelled anti-mouse antibody as detection. The blocking is done with BSA.
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