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Trypsin inhibitor

Name: Trypsin inhibitor
Brand: SIGMA
Price: 79.7 USD
Availability: InStock

lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

Synonym(s):

Kunitz Inhibitor

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Size/SKU	Availability	Price
5 mg	Check Cart for Availability	$79.70
10 mg	Check Cart for Availability	$161.00
25 mg	Check Cart for Availability	$398.00

About This Item

CAS Number:

9035-81-8

UNSPSC Code:

12352204

NACRES:

NA.77

EC Number:

232-906-9

MDL number:

MFCD00082102

$79.70

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Properties

Product Name

Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

biological source

Glycine max (soybean)

Quality Segment

200

product line

BioUltra

assay

≥95% (Kunitz inhibitor, SDS-PAGE)

form

lyophilized powder

input

plant, fungi and insect sample(s)

storage temp.

2-8°C

Description

General description

Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.

Application

Trypsin Inhibitor from Glycine max (soybean) has been used:

as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris
as a standard to compare the trypsin inhibitory activity of the purified protein
to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography
as an trypsin inhibitor

Biochem/physiol Actions

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x10⁸.

Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.

Preparation Note

Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Analysis Note

One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

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This Item	T9767	T6414	P8193
Sigma-Aldrich T2327 Trypsin Inhibitor from Glycine max (soybean)	Sigma-Aldrich T9767 Trypsin inhibitor from Glycine max (soybean)	Sigma-Aldrich T6414 Trypsin inhibitor from Glycine max (soybean)	Sigma-Aldrich P8193 L-α-Phosphatidylethanolamine from Glycine max (soybean)
description BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)	description BioReagent, For use as a marker in SDS-PAGE	description solution, sterile-filtered, suitable for, suitable for cell culture	description Type IV, ≥98% (TLC), lyophilized powder
assay ≥95% (Kunitz inhibitor, SDS-PAGE)	assay -	assay -	assay ≥98% (TLC)
biological source Glycine max (soybean)	biological source Glycine max (soybean)	biological source Glycine max (soybean)	biological source soybean
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200
form lyophilized powder	form powder	form solution	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
product line BioUltra	product line BioReagent	product line BioReagent	product line -

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Precautionary Statements

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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