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T2400

Tropomyosin from porcine muscle

lyophilized powder (contains Tris buffer salts)

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1 mg

Available to ship TODAYfromMILWAUKEE

$198.00

About This Item

CAS Number:
9067-56-5
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00132526
Form:
lyophilized powder (contains Tris buffer salts)
Assay:
≥70% protein basis (biuret)
Biological source:
Porcine muscle

$198.00

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biological source

Porcine muscle

Quality Segment

200

assay

≥70% protein basis (biuret)

form

lyophilized powder (contains Tris buffer salts)

technique(s)

electrophoresis: suitable

storage temp.

−20°C

General description

Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells.

Application

Tropomyosin from porcine muscle has been used:

  • as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay
  • in analyzing the anti-adhesive properties of fish tropomyosins
  • in far Western blot analysis

Biochem/physiol Actions

Dimeric protein that assumes an α-helical coiled coil conformation. In the absence of Ca2+, inhibits muscle contractility by blocking the myosin binding sites on actin.
Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin.
Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin.

Analysis Note

SDS electrophoresis shows two major bands.

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Tropomyosin from porcine muscle lyophilized powder (contains Tris buffer salts)

Sigma-Aldrich

T2400

Tropomyosin from porcine muscle

Actin from rabbit muscle ≥85% (SDS-PAGE), lyophilized powder

Sigma-Aldrich

A2522

Actin from rabbit muscle

Albumin from porcine serum lyophilized powder, 96-99% (agarose gel electrophoresis)

Sigma-Aldrich

A2764

Albumin from porcine serum

Phosphorylase b from rabbit muscle lyophilized powder, ≥20 units/mg protein, 2× crystallization

Sigma-Aldrich

P6635

Phosphorylase b from rabbit muscle

description

lyophilized powder (contains Tris buffer salts)

description

≥85% (SDS-PAGE), lyophilized powder

description

lyophilized powder, 96-99% (agarose gel electrophoresis)

description

lyophilized powder, ≥20 units/mg protein, 2× crystallization

assay

≥70% protein basis (biuret)

assay

≥85% (SDS-PAGE)

assay

96-99% (agarose gel electrophoresis)

assay

-

biological source

Porcine muscle

biological source

rabbit

biological source

Porcine

biological source

rabbit muscle

technique(s)

electrophoresis: suitable

technique(s)

immunoblotting: suitable

technique(s)

ELISA: suitable

technique(s)

mass spectrometry (MS): suitable

form

lyophilized powder (contains Tris buffer salts)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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