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T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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Size/SKUAvailabilityPrice
10 mg
0 left in stock
$1220.00
25 mg

Estimated to ship TODAYfromSAINT LOUIS

$1,220.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-774-2
MDL number:
EC Number:
Specific activity:
~10,000 units/mg protein

$1220.00


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type

Type I

Quality Segment

form

ammonium sulfate suspension

specific activity

~10,000 units/mg protein

mol wt

53 kDa by equilibrium centrifugation

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

storage temp.

2-8°C

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Physical form

Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

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description

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

description

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

description

for use with Fructose Assay Kit FA-20

description

ammonium sulfate suspension, ≥500 units/mg protein

specific activity

~10,000 units/mg protein

specific activity

≥400 units/mg protein (biuret)

specific activity

-

specific activity

≥500 units/mg protein

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

-

form

ammonium sulfate suspension

mol wt

53 kDa by equilibrium centrifugation

mol wt

tetramer 119,500 Da±600

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

foreign activity

-

foreign activity

-

foreign activity

Glyceraldehyde-3-phosphate dehydrogenase ≤0.1%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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