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T2807

Thymidine Phosphorylase, recombinant from Escherichia coli

recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL

Synonym(s):

Thymidine:orthophosphate deoxy-D-ribosyltransferase

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$757.00

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About This Item

CAS Number:
9030-23-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
2.4.2.4 (BRENDA, IUBMB)
MDL number:
MFCD00132426
Recombinant:
expressed in E. coli
Concentration:
≥500 units/mL

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Product Name

Thymidine Phosphorylase, recombinant from Escherichia coli, recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL

recombinant

expressed in E. coli

form

buffered aqueous solution

concentration

≥500 units/mL

UniProt accession no.

P07650

storage temp.

2-8°C

Quality Level

300

Gene Information

Escherichia coli K12 ... deoA(948901)

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This Item
T6632SRE0006T7915
Thymidine Phosphorylase, recombinant from Escherichia coli recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL

Sigma-Aldrich

T2807

Thymidine Phosphorylase, recombinant from Escherichia coli

Thymidine Phosphorylase, recombinant from Escherichia coli recombinant, expressed in E. coli, buffered aqueous solution, ≥900 units/mL, 0.2 μm filtered

Sigma-Aldrich

T6632

Thymidine Phosphorylase, recombinant from Escherichia coli

Thymidine Phosphorylase, recombinant from Escherichia coli recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL

Sigma-Aldrich

SRE0006

Thymidine Phosphorylase, recombinant from Escherichia coli

Thioredoxin Reductase from Escherichia coli ammonium sulfate suspension, >25 units/mg protein (Bradford)

Sigma-Aldrich

T7915

Thioredoxin Reductase from Escherichia coli

Gene Information

Escherichia coli K12 ... deoA(948901)

Gene Information

Escherichia coli K12 ... deoA(948901)

Gene Information

Escherichia coli ... deoA(948901)

Gene Information

Escherichia coli K12 ... trxB(949054)

concentration

≥500 units/mL

concentration

≥900 units/mL

concentration

≥500 units/mL

concentration

-

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

ammonium sulfate suspension

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

UniProt accession no.

P07650

UniProt accession no.

P07650

UniProt accession no.

P07650

UniProt accession no.

P0A9P4

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Thymidine phosphorylase has been used in a study to assess TAS-102 treatment in patients with advanced solid tumors. [1] Thymidine phosphorylase has also been used in a study to investigate antitumor effects of the FP3 protein on patient-derived tumor tissue xenograft models of primary colon carcinoma. [2]

Biochem/physiol Actions

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway.
An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

General description

Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation. [3]

Other Notes

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Physical form

Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin

Preparation Note

Cloned from E. coli and produced in overexpressing E. coli

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000515714
0000515713
0000515714
0000515713
0000474648

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Alexandra Giatromanolaki et al.
Cancer biology & therapy, 13(13), 1284-1289 (2012-08-17)
Tumor-associated stroma (TAS) is not simply a supporting element for cancer cells, but plays an important role in tumor growth, invasion and metastasis. Changes on the level of stromal constituents, such as loss of Caveolin-1 and increased thymidine phosphorylase (TP)
Michelle Levene et al.
Toxicological sciences : an official journal of the Society of Toxicology, 131(1), 311-324 (2012-09-15)
Erythrocyte-encapsulated thymidine phosphorylase (EE-TP) is currently under development as an enzyme replacement therapy for mitochondrial neurogastrointestinal encephalomyopathy (MNGIE), an autosomal recessive disorder caused by a deficiency of thymidine phosphorylase. The rationale for the development of EE-TP is based on the
Ketao Jin et al.
Cancer biology & therapy, 13(9), 737-744 (2012-05-24)
FP3 is an engineered protein which contains the extracellular domain 2 of VEGF receptor 1 (Flt-1) and extracellular domain 3 and 4 of VEGF receptor 2 (Flk-1, KDR) fused to the Fc portion of human immunoglobulin G 1. Previous studies
Hong-Yun Zhao et al.
Anti-cancer drugs, 23(5), 534-542 (2012-04-07)
The aim of the present study was to investigate the gene expression of biomarkers associated with the sensitivity to fluoropyrimidine and taxanes in recurrent/advanced breast cancer patients treated with first-line capecitabine chemotherapy. We evaluated the clinicopathological/prognostic significance of thymidylate synthase
T Doi et al.
British journal of cancer, 107(3), 429-434 (2012-06-28)
TAS-102 consists of α, α, α-trifluorothymidine (TFT) and an inhibitor of thymidine phosphorylase (TPI). We conducted a dose-escalation phase I study in Japanese patients with advanced solid tumours. TAS-102 was administered twice daily on days 1-5 and days 8-12 in
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