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T4299

Trypsin-EDTA solution

1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA, 4Na per ml in Dulbecco′s PBS without calcium and magnesium

Synonym(s):

Cocoonase, Tryptar, Tryptase

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Size/SKUAvailabilityPrice
100 mL
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$99.50

About This Item

UNSPSC Code:
12352204
NACRES:
NA.75
MDL number:
MFCD00130286
Biological source:
Porcine pancreas
Concentration:
1 ×

$99.50

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biological source

Porcine pancreas

Quality Segment

300

sterility

sterile-filtered

product line

BioReagent

form

solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

impurities

Porcine parvovirus, none detected (9 CFR)

pH

7.0-7.6

shipped in

dry ice

storage temp.

−20°C

Application

The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It can be used with endothelial cell cultures.

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Other Notes

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Disclaimer

This product is stored frozen between -10 and -40°C. Repeated cycles of freezing and thawing should be avoided.

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This Item
T4174T4049T3924
Trypsin-EDTA solution 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA, 4Na per ml in Dulbecco′s PBS without calcium and magnesium

Sigma-Aldrich

T4299

Trypsin-EDTA solution

Sigma-Aldrich

Sigma-Aldrich

T4174

Trypsin-EDTA solution

Sigma-Aldrich

Sigma-Aldrich

T4049

Trypsin-EDTA solution

Sigma-Aldrich

Sigma-Aldrich

T3924

Trypsin-EDTA solution

description

1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA, 4Na per ml in Dulbecco′s PBS without calcium and magnesium

description

10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

description

0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

description

1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

biological source

Porcine pancreas

biological source

Porcine

biological source

Porcine

biological source

Porcine

concentration

1 ×

concentration

10 ×

concentration

0.25%

concentration

1 ×

form

solution

form

solution

form

solution

form

solution

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

pH

7.0-7.6

pH

7.0-7.6

pH

7.0-7.6

pH

7.0-8.0

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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