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Thrombin from human plasma

Name: Thrombin from human plasma
Brand: SIGMA
Price: 338 USD
Availability: InStock

lyophilized powder, 1500-3500 NIH units/mg protein (E1%/280, 18.3), suitable for cell culture

Synonym(s):

Factor IIa

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Size/SKU	Availability	Price
100 units	Available to ship TODAYfromMILWAUKEE	$338.00

About This Item

CAS Number:

9002-04-4

UNSPSC Code:

12352202

NACRES:

NA.75

EC Number:

232-648-7

MDL number:

MFCD00082072

EC Number:

3.4.21.5 (BRENDA, IUBMB)

Specific activity:

1500-3500 NIH units/mg protein (E1%/280, 18.3)

Biological source:

human plasma

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Properties

biological source

human plasma

Quality Segment

200

sterility

sterile

form

lyophilized powder

specific activity

1500-3500 NIH units/mg protein (E1%/280, 18.3)

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, tested negative

UniProt accession no.

P00734

storage temp.

−20°C

Gene Information

human ... F2(2147)

Description

General description

Thrombin is produced from the proteolytic cleavage of inactive prothrombin in the liver. The prothrombin gene is mapped to human chromosome 11p11.2. It comprises of A and B catalytic domain, recognition domain and insertion loops. The active site residues comprise the catalytic tetrad, (histidine 57, aspartate 102, serine 195 and serine 214).

Application

Thrombin from human plasma has been used:

as a medium supplement for the pre-treatment of endothelial cell culture prior to confocal microscopy and enzyme linked immunosorbent assay (ELISA)
in the gelatinization of mesenchymal stem cells (MSCs) for preparing fibrin–MSC construct
for screening serine protease inhibitor, OGTI from frog skin secretion

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles in the brain may contribute to the aggregation of τ protein and pathophysiology of Alzheimer disease.

Preparation Note

When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.

Analysis Note

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Show Differences

1 of 1

This Item	T7009	T1063	T8885
Sigma-Aldrich T4393 Thrombin from human plasma	Sigma-Aldrich T7009 Thrombin from human plasma	Sigma-Aldrich T1063 Thrombin from human plasma	Sigma-Aldrich T8885 Thrombin from human plasma
description lyophilized powder, 1500-3500 NIH units/mg protein (E1%/280, 18.3), suitable for cell culture	description lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)	description lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)	description lyophilized powder, Suitable for routine use in the thrombin time test
biological source human plasma	biological source -	biological source -	biological source human plasma
Gene Information human ... F2(2147)	Gene Information human ... F2(2147)	Gene Information human ... F2(2147)	Gene Information human ... F2(2147)
specific activity 1500-3500 NIH units/mg protein (E1%/280, 18.3)	specific activity ≥1,000 NIH units/mg protein (E1%/280, 18.3)	specific activity ≥2800 NIH units/mg protein (E1%/280, 18.3)	specific activity -
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Danger

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

target_organs

Respiratory system

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

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