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T4904

Tris-Glycine Buffer 10× Concentrate

Synonym(s):

running buffer

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Size/SKUAvailabilityPrice
1 L

Available to ship onOctober 21, 2026fromMILWAUKEE

$155.00

About This Item

UNSPSC Code:
41105323
EC Number:
232-650-8
NACRES:
NA.25
eCl@ss:
32110600

$155.00

Available to ship onOctober 21, 2026Details

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sterility

0.2 μm filtered

Quality Segment

foreign activity

Protease, none detected

storage temp.

2-8°C

Application

Tris-Glycine buffer 10× concentrate has been used as a transfer buffer for Western blotting.

Biochem/physiol Actions

For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.

Other Notes

0.25 M Tris, 1.92 M glycine, pH approx. 8.3
Contains no methanol.

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Sigma-Aldrich

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Tris-Glycine Buffer pH 8.3, pHast Pack™, powder

Sigma-Aldrich

PPB015

Tris-Glycine Buffer

Protein-Concentrate Kit (Micro) Protein-Concentrate Kit (Micro) is suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays & other applications.

Millipore

2100

Protein-Concentrate Kit (Micro)

description

-

description

-

description

pH 8.3, pHast Pack™, powder

description

Protein-Concentrate Kit (Micro) is suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays & other applications.

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

-

foreign activity

Protease, none detected

foreign activity

-

foreign activity

NICKase, none detected, Endonuclease , DNase, none detected, Exonuclease, none detected, Protease, none detected, RNase, none detected

foreign activity

-

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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