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Trypsin from porcine pancreas

Name: Trypsin from porcine pancreas
Brand: SIGMA
Price: 73.8 USD
Availability: InStock

lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

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Size/SKU	Availability	Price
500 mg	Available to ship TODAYfromMILWAUKEE	$73.80

About This Item

CAS Number:

9002-07-7

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

232-650-8

MDL number:

MFCD00082094

EC Number:

3.4.21.4 (BRENDA, IUBMB)

Specific activity:

1,000-2,000 BAEE units/mg solid

Biological source:

Porcine pancreas

Concentration:

25 mg/mL

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Properties

biological source

Porcine pancreas

Quality Segment

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

specific activity

1,000-2,000 BAEE units/mg solid

mol wt

23.8 kDa

concentration

25 mg/mL

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

Description

Application

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Preparation Note

This product is a lyophilized powder soluble in Hank′s Balanced Salt Solution at 25 mg/mL.
For applications that involve EDTA, solubilizing trypsin should be done with a buffered salt solution contaiing no Ca²⁺ or Mg²⁺.

Other Notes

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. One BTEE unit = 320 ATEE units. Reaction volume = 3.2 mL (1 cm light path).

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Disclaimer

Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca²⁺ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.

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This Item	T4674	T4549	T4424
Sigma-Aldrich T5266 Trypsin from porcine pancreas	Sigma-Aldrich T4674 Trypsin solution from porcine pancreas	Sigma-Aldrich T4549 Trypsin solution from porcine pancreas	Sigma-Aldrich T4424 Trypsin solution from porcine pancreas
description lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid	description sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red	description sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride	description 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
specific activity 1,000-2,000 BAEE units/mg solid	specific activity -	specific activity -	specific activity -
biological source Porcine pancreas	biological source Porcine pancreas	biological source Porcine	biological source Porcine pancreas
form lyophilized powder	form solution	form solution	form solution
concentration 25 mg/mL	concentration -	concentration -	concentration 1 ×
shipped in wet ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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