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T5694

TRAIL human

recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder

Synonym(s):

Apo2 Ligand, Apo2L, TL2, TNF-related apoptosis-inducing ligand

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Size/SKUAvailabilityPrice
10 μg
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$677.00

About This Item

CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
Form:
lyophilized powder
Assay:
>97% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in NSO cells
Mol wt:
24 kDa by SDS-PAGE, predicted mol wt ~21 kDa

$677.00


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biological source

human

Quality Segment

recombinant

expressed in NSO cells

description

N-terminal 6X histidine-tagged extracellular domain

assay

>97% (SDS-PAGE)

form

lyophilized powder

mol wt

24 kDa by SDS-PAGE, predicted mol wt ~21 kDa

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TNFSF10(8743)

Application

TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line.

Biochem/physiol Actions

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells.
Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D.

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T9701GF092SRP8024
description

recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE and HPLC), lyophilized powder

description

Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells.

description

recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

recombinant

expressed in NSO cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

technique(s)

cell culture | mammalian: suitable

technique(s)

immunofluorescence: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

activity assay: suitable, transfection: suitable, cell culture | mammalian: suitable

assay

>97% (SDS-PAGE)

assay

≥98% (SDS-PAGE and HPLC)

assay

>98% (SDS-PAGE and HPLC)

assay

>98% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

form

lyophilized powder

form

lyophilized powder

form

solid

form

liquid

mol wt

predicted mol wt ~21 kDa, 24 kDa by SDS-PAGE

mol wt

predicted mol wt ~21 kDa, 19.6 kDa

mol wt

19.6 kDa

mol wt

~26 kDa


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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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