Anti-Troponin T antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the JLT-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified Troponin T from rabbit skeletal muscle. These papers would provide more details about the immunogen sequence:
1. Lin JJ., et al., Monoclonal Antibodies and Functional Cell lines, 119–51. NY: Plenum Publishing (1984).
2. Briggs MM., et al., J Muscle Res Cell Motil., 8, 1-12 (1987).
T6277
Anti-Troponin T antibody, Mouse monoclonal
clone JLT-12, purified from hybridoma cell culture
Synonym(s):
Anti-ANM, Anti-NEM5, Anti-STNT, Anti-TNT, Anti-TNTS
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100 μL | Check Cart for Availability | $552.00 |
0.2 mL | Check Cart for Availability | $727.00 $617.95 |
About This Item
Conjugate:
unconjugated
Clone:
JLT-12, monoclonal
Application:
IHC, WB
Citations:
87
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
JLT-12, monoclonal
contains
15 mM sodium azide
species reactivity
chicken, rat, bovine, rabbit
technique(s)
immunohistochemistry: suitable, western blot: 1:200 using rabbit total skeletal muscle extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Tnnt1(171409), Tnnt2(24837), Tnnt3(24838)
General description
Monoclonal Anti-Troponin T (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Troponin T is a vital subunit of troponin. Troponin T is a comma or rod shaped protein with a length of 185-205 Å. Troponin T is positioned in the actin helix groove and is extended along the filament of troponin. Life span of serum troponin T is 120 minutes. N- terminal end of the protein is enriched in negatively charged residues and C- terminal end is enriched with positively charged residues. This uneven distribution of charges in troponin T facilitates its aggregation at physiological salt concentration. In humans, cardiac troponin T exists in four isoforms, out of which three are expressed in the fetus and one isoform is expressed in the adult heart.
Immunogen
Troponin T from rabbit skeletal muscle.
Application
Monoclonal Anti-Troponin T antibody produced in mouse has been used in immunostaining.
Biochem/physiol Actions
Troponin T is a subunit of troponin, which facilitates binding of troponin to tropomyosin. It also plays a crucial role in muscle contraction. Cardiac troponin T acts as a potential marker for the detection of acute myocardial infarction (MI). Apart from facilitating binding of troponin components to the actin-tropomyosin filament, it is has an essential role in the regulation of actomyosin ATPase activity. Cardiac troponin T gene mutation leads to hypertrophic cardiomyopathy.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description clone JLT-12, purified from hybridoma cell culture | description clone TM311, ascites fluid | description clone TH-16, purified from hybridoma cell culture | description clone HM-1, ascites fluid |
| clone JLT-12, monoclonal | clone TM311, monoclonal | clone TH-16 | clone HM-1, monoclonal |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form purified from hybridoma cell culture | antibody form ascites fluid |
| species reactivity chicken, rat, bovine, rabbit | species reactivity pig, chicken, bovine, rabbit, mouse, hamster, rat, human | species reactivity rat, sheep, human, monkey, bovine, rabbit, guinea pig | species reactivity mouse, rat |
| Gene Information rat ... Tnnt1(171409), Tnnt2(24837), Tnnt3(24838) | Gene Information bovine ... TPM1(281544) | Gene Information human ... TH(7054) | Gene Information mouse ... Mtap1a(17754) |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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bonjour , est ce que vous avez une information sur la séquence qui est ciblée par l'anticorps (T6277) ? merci
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