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T6451

Anti-Twist1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Twist homolog 1 (acrocephalosyndactyly 3, Saethre-Chotzen syndrome), Anti-Twist related protein 1 (H-twist)

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Size/SKUAvailabilityPrice
200 μL
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$614.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD08705531
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB CL
Citations:
25

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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~26 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using human osteosarcoma MG-63 cells, western blot (chemiluminescent): 0.5-1 μg/mL using human 293T cells expressing recombinant human Twist1

UniProt accession no.

Q15672

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TWIST1(7291)
mouse ... Twist1(22160)
rat ... Twist1(85489)

General description

Twist is encoded by the gene mapped to human chromosome 7p21.1. The encoded protein is a basic helix-loop-helix (bHLH) transcription factor. Twist 1 belongs to the Twist gene family and is mainly expressed in the suture mesenchyme.

Immunogen

synthetic peptide corresponding to amino acids 12-27 of human Twist1 with a C-terminal added cysteine, conjugated to KLH. The corresponding peptide sequence is identical in both rat and mouse.

Application

Anti-Twist1 antibody is suitable for use in detecting Twist1 via in immunochemical applications such as immunoblotting (~26 kDa) and immunofluorescence.
Immunoblotting: a working antibody concentration of 0.5-1 mg/mL is recommended using extracts of human 293T cells expressing recombinant human Twist1 and a chemiluminescent detection reagent.
Anti-Twist1 antibody produced in rabbit has been used in:
  • immunohistochemistry
  • immunofluorescence
  • immunoblotting
  • chromatin immunoprecipitation (ChIP)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Biochem/physiol Actions

Anti-Twist1 antibody recognizes human Twist1. Detection of the Twist1 band by immunoblotting is specifically inhibited with the immunizing peptide.
Twist is required for mesoderm formation. Twist 1 controls embryonic morphogenesis. It also plays a key role in metastasis by inducing an epithelial-mesenchymal transition (EMT). During an EMT, Twist stimulates the expression of mesenchymal markers, such as fibronectin and N-cadherin. In addition, it also blocks E-cadherin expression inhibiting cell-cell adhesion and therefore inducing infiltrative tumor growth in several human carcinomas. Twist1 is required in head mesenchyme for cranial neural tube morphogenesis in mice. Twist1 is involved in osteoblast differentiation and maturation. Increased expression of Twist 1 in breast cancer cells induce angiogenesis and leads to chromosomal instability. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome, an autosomal dominant defect characterized by minor skull and limb anomalies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Twist1 antibody produced in rabbit ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Monoclonal Anti-TWIST1, (C-terminal) antibody produced in mouse

description

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

description

clone 3E11, purified immunoglobulin, buffered aqueous solution

description

purified immunoglobulin, buffered aqueous solution

description

clone 2F8, purified immunoglobulin, buffered aqueous solution

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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