1ug=1.1x10e5 units.
T6674
Tumor Necrosis Factor-α human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym(s):
hTNF-α, TNF-α
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Check Cart for Availability | $236.00 |
5 x 10 μg | Check Cart for Availability | $1,910.00 |
About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
powder
Mol wt:
~17.4 kDa
Impurities:
<1 EU/μg
$236.00
Check Cart for Availability
Product Name
Tumor Necrosis Factor-α human, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
powder
potency
0.02-0.3 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
~17.4 kDa
packaging
pkg of 5x10 μg, pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TNF(7124)
Application
TNF-α include stimulating growth of human fibroblasts and other cell lines activating polymorphonuclear neutrophils and osteoclasts and inducing interleukin-1, prostaglandin E2, and collagenase production.
Tumor Necrosis Factor-α (TNFα) human has been used:
- to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells
- to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression
- to induce death-receptor-mediated apoptosis in HeLa cells.
Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in anti-tumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.
Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.
Physical form
Lyophilized from a 0.2 μm-filtered buffer solution containing 3mM Tris-HCl and 5% trehalose.
Analysis Note
The biological activity of TNF-α is measured by the cytolysis of murine L929 cells.
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This Item | |||
|---|---|---|---|
| description TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture | description TNF-β, recombinant, expressed in E. coli, powder, suitable for cell culture | description TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture | description recombinant, expressed in E. coli, ≥80% (SDS-PAGE) |
| assay ≥97% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay ≥98% (SDS-PAGE and HPLC) | assay ≥80% (SDS-PAGE) |
| biological source human | biological source human | biological source rat | biological source human |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| form powder | form powder | form powder | form frozen liquid |
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What are the units/mg of Product T6674, Tumor Necrosis Factor-α human?
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What is the EC50 of Product T6674, Tumor Necrosis Factor-α human?
1 answer-
Bioactivity(cell inhibition, L929 cells) is expressed by an EC50 of 0.02 to 0.05 ng/mL for T6674. EC50 (equivalent concentration) is the same as ED50 (equivalent dose, cytotoxicity, L929 cells) used for the bioactivity specification for T0157, Tumor Necrosis Factor-alpha, human recombinant expressed in Yeast.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solubility of this Product T6674, Tumor Necrosis Factor-α human?
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This can be dissolved at 0.1 to 1.0 mg/ml in saline or buffer, stabilized with 0.1 to 1%BSA.
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